Science子刊:大肠杆菌群体感应可能与糖代谢有关

细菌通过分泌自诱导物(AI)介导群体感应,许多细菌物种分泌AI-2;
细菌通过lsr操纵子感知AI-2,lsr操纵子中包括AI-2转运蛋白LsrACDB及LsrK激酶;
HPr是大肠杆菌糖磷酸转移酶系统的核心组分,依赖于磷酸烯醇丙酮酸,可与LsrK激酶被共同纯化;
解析LsrK/HPr复合物的共结晶结构,发现与HPr结合后,LsrK的活性被抑制;
提示细菌的群体感应活性与糖代谢间的关联。
延伸阅读
Science advances [IF:11.511]

Evidence of link between quorum sensing and sugar metabolism in Escherichia coli revealed via cocrystal structures of LsrK and HPr

通过解析LsrK及HPr的共结晶结构揭示大肠杆菌的群体感应与糖代谢之间的关联

2018-06-01, Article, 10.1126/sciadv.aar7063more

Abstract:
Quorum sensing (QS), a bacterial process that regulates population-scale behavior, is mediated by small signaling molecules, called autoinducers (AIs), that are secreted and perceived, modulating a “collective” phenotype. Because the autoinducer AI-2 is secreted by a wide variety of bacterial species, its “perception” cues bacterial behavior. This response is mediated by the lsr (LuxS-regulated) operon that includes the AI-2 transporter LsrACDB and the kinase LsrK. We report that HPr, a phosphocarrier protein central to the sugar phosphotransferase system of Escherichia coli, copurifies with LsrK. Cocrystal structures of an LsrK/HPr complex were determined, and the effects of HPr and phosphorylated HPr on LsrK activity were assessed. LsrK activity is inhibited when bound to HPr, revealing new linkages between QS activity and sugar metabolism. These findings help shed new light on the abilities of bacteria to rapidly respond to changing nutrient levels at the population scale. They also suggest new means of manipulating QS activity among bacteria and within various niches.

First Authors:
Jung-Hye Ha,Pricila Hauk

Correspondence Authors:
Herman O Sintim,William E Bentley,Kyoung-Seok Ryu

All Authors:
Jung-Hye Ha,Pricila Hauk,Kun Cho,Yumi Eo,Xiaochu Ma,Kristina Stephens,Soyoung Cha,Migyeong Jeong,Jeong-Yong Suh,Herman O Sintim,William E Bentley,Kyoung-Seok Ryu