SR:IBD病人的肠道上皮与免疫细胞如何互作?
  • IBD与肠道上皮细胞(IEC)的功能受损相关,且伴随着炎症细胞向固有层的渗透;
  • 在模拟IBD的共培养系统中研究人体IEC与巨噬细胞的相互作用;
  • IEC分别与其它IEC及巨噬细胞建立了功能性间隙连接;
  • IEC上的连接蛋白Cx26与Cx43在炎症状态下高表达;
  • Cx43相关连接复合物伴侣:E-钙黏蛋白、ZO-1及β连环蛋白表达下调。
主编推荐语
蓝灿辉 | 热心肠先生
在体外共培养系统中研究人体上皮和巨噬细胞的相互作用,以研究IBD,很好的思路,建议看看。
关键字
延伸阅读本研究的原文信息和链接出处,以及相关解读和评论文章。欢迎读者朋友们推荐!
图片
Scientific Reports [IF:3.998]

Cross-talk between intestinal epithelial cells and immune cells in inflammatory bowel disease

IBD中肠道上皮细胞及免疫细胞的互作

10.1038/srep29783

2016-07-15, Article

Abstract & Authors:展开

Abstract:收起
Inflammatory bowel disease (IBD) involves functional impairment of intestinal epithelial cells (IECs), concomitant with the infiltration of the lamina propria by inflammatory cells. We explored the reciprocal paracrine and direct interaction between human IECs and macrophages (MΦ) in a co-culture system that mimics some aspects of IBD. We investigated the expression of intercellular junctional proteins in cultured IECs under inflammatory conditions and in tissues from IBD patients. IECs establish functional gap junctions with IECs and MΦ, respectively. Connexin (Cx26) and Cx43 expression in cultured IECs is augmented under inflammatory conditions; while, Cx43-associated junctional complexes partners, E-cadherin, ZO-1, and β-catenin expression is decreased. The expression of Cx26 and Cx43 in IBD tissues is redistributed to the basal membrane of IEC, which is associated with decrease in junctional complex proteins' expression, collagen type IV expression and infiltration of MΦ. These data support the notion that the combination of paracrine and hetero-cellular communication between IECs and MΦs may regulate epithelial cell function through the establishment of junctional complexes between inflammatory cells and IECs, which ultimately contribute to the dys-regulation of intestinal epithelial barrier.

First Authors:
Sara Al-Ghadban

Correspondence Authors:
Marwan E El-Sabban

All Authors:
Sara Al-Ghadban,Samira Kaissi,Fadia R Homaidan,Hassan Y Naim,Marwan E El-Sabban

评论