• IBD与肠道上皮细胞(IEC)的功能受损相关,且伴随着炎症细胞向固有层的渗透;
  • 在模拟IBD的共培养系统中研究人体IEC与巨噬细胞的相互作用;
  • IEC分别与其它IEC及巨噬细胞建立了功能性间隙连接;
  • IEC上的连接蛋白Cx26与Cx43在炎症状态下高表达;
  • Cx43相关连接复合物伴侣:E-钙黏蛋白、ZO-1及β连环蛋白表达下调。
蓝灿辉 | 热心肠先生
Scientific Reports [IF:3.998]

Cross-talk between intestinal epithelial cells and immune cells in inflammatory bowel disease



2016-07-15, Article

Abstract & Authors:展开

Inflammatory bowel disease (IBD) involves functional impairment of intestinal epithelial cells (IECs), concomitant with the infiltration of the lamina propria by inflammatory cells. We explored the reciprocal paracrine and direct interaction between human IECs and macrophages (MΦ) in a co-culture system that mimics some aspects of IBD. We investigated the expression of intercellular junctional proteins in cultured IECs under inflammatory conditions and in tissues from IBD patients. IECs establish functional gap junctions with IECs and MΦ, respectively. Connexin (Cx26) and Cx43 expression in cultured IECs is augmented under inflammatory conditions; while, Cx43-associated junctional complexes partners, E-cadherin, ZO-1, and β-catenin expression is decreased. The expression of Cx26 and Cx43 in IBD tissues is redistributed to the basal membrane of IEC, which is associated with decrease in junctional complex proteins' expression, collagen type IV expression and infiltration of MΦ. These data support the notion that the combination of paracrine and hetero-cellular communication between IECs and MΦs may regulate epithelial cell function through the establishment of junctional complexes between inflammatory cells and IECs, which ultimately contribute to the dys-regulation of intestinal epithelial barrier.

First Authors:
Sara Al-Ghadban

Correspondence Authors:
Marwan E El-Sabban

All Authors:
Sara Al-Ghadban,Samira Kaissi,Fadia R Homaidan,Hassan Y Naim,Marwan E El-Sabban