Science:病原菌激活植物内生菌群的抑病功能
  • 生活在植物内部的微生物可以促进植物的生长和健康,但基因组和功能多样性仍不清楚;
  • 宏基因组学和网络推论表明,植物根部的真菌感染在根内富集了几丁质菊科和黄杆菌科,以及几丁质酶基因和编码未知核糖体肽合成酶(NRPSs)和聚酮化合物合酶(PKSs)的生物合成基因簇;
  • 菌株级别合成的几丁质和黄杆菌的菌群可以持续抑制真菌根部疾病;
  • 菌株的基因突变实验表明新发现的黄杆菌中的NRPS-PKS基因簇在内生菌群抑制疾病中起重要作用。
主编推荐语
刘永鑫-中科院-宏基因组
抑病型土壤的研究主要集中在特异性抑病土壤上,目前已经有很多高水平文章解析过抑病型微生物群落,这篇Science无疑是最新和最全的一篇。从内容上来,描述了抑病型土壤的群落结构和功能;得到了候选功能菌株,并通过培养组得到了大量的分离株,并开展菌株功能验证;而后进行功能基因的筛选,敲除和验证,最终形成了一个完美的故事。确实是本领域研究的学习模板,并值得我们重现这篇文章的分析过程。 这篇文章关注的是抑病型微生物群落的解析,对于抑病型微生物群落的形成也十分重要,那么内生菌群是如何被植物招募并进入根系内部的呢?是否根系分泌物在这个过程中也起着重要作用?相信这将是很有意思的故事。
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Science [IF:41.845]

Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome

病原菌介导植物内生菌群抑病功能的激活

10.1126/science.aaw9285

2019-11-01, Article

Abstract & Authors:展开

Abstract:收起
Microorganisms living inside plants can promote plant growth and health, but their genomic and functional diversity remain largely elusive. Here, metagenomics and network inference show that fungal infection of plant roots enriched for Chitinophagaceae and Flavobacteriaceae in the root endosphere and for chitinase genes and various unknown biosynthetic gene clusters encoding the production of nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). After strain-level genome reconstruction, a consortium of Chitinophaga and Flavobacterium was designed that consistently suppressed fungal root disease. Site-directed mutagenesis then revealed that a previously unidentified NRPS-PKS gene cluster from Flavobacterium was essential for disease suppression by the endophytic consortium. Our results highlight that endophytic root microbiomes harbor a wealth of as yet unknown functional traits that, in concert, can protect the plant inside out

First Authors:
Víctor J Carrión,Juan Perez-Jaramillo,Viviane Cordovez,Vittorio Tracanna

Correspondence Authors:
Marnix H Medema,Jos M Raaijmakers

All Authors:
Víctor J Carrión,Juan Perez-Jaramillo,Viviane Cordovez,Vittorio Tracanna,Mattias de Hollander,Daniel Ruiz-Buck,Lucas W Mendes,Wilfred FJ van Ijcken,Ruth Gomez-Exposito,Somayah S Elsayed,Prarthana Mohanraju,Adini Arifah,John van der Oost,Joseph N Paulson,Rodrigo Mendes,Gilles P van Wezel,Marnix H Medema,Jos M Raaijmakers

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Nature Reviews Microbiology期刊

At the root of the problem

Andrea Du Toit,2019-11-13

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宏基因组微信公众号

Science:病原菌激活植物内生菌群的抑病功能

2020-03-14

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Science期刊

A layered defense against plant pathogens

Susannah G. Tringe,2019-10-31

点评:Science同期配发的评论解读文章。

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