膳食纤维改变结肠粘蛋白O-糖基化及其与肠道菌群的互作
创作:徐笑 审核:szx 2020年10月11日
  • 高脂饮食组小鼠糖耐量受损,肝脏蛋白组、肠道菌群组成和短链脂肪酸发生改变;
  • 膳食纤维摄入改变肠道菌群组成(可降解纤维的细菌科的相对丰度增加)和短链脂肪酸产生(丙酸盐浓度增加);
  • 正常饮食组和高脂饮食组小鼠的结肠粘蛋白O-糖基化相似,而膳食纤维干预可导致O-糖基化模式的改变,以抑制粘液层降解;
  • 特定肠道细菌与粘蛋白O-糖基化结构相关,例如Parabacteroides distasonis的相对丰度与含有一个末端岩藻糖多糖结构呈正相关。
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szx
结肠粘液层中粘蛋白高度O-糖基化,在介导宿主-肠道菌群互作中发挥关键作用,但饮食变化如何影响结肠粘蛋白O-糖基化及其与菌群的互作尚未明确。Gut Microbes上发表的一项最新研究,发现膳食纤维可诱导小鼠的结肠粘蛋白O-糖基化发生变化,促进特定糖基化模式的形成以抑制粘液层降解。另外,膳食纤维摄入可改变小鼠的肠道菌群组成,且特定肠道细菌与结肠粘蛋白O-糖基化结构密切相关,提示膳食纤维可改变结肠粘蛋白与肠道菌群的互作。
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Gut Microbes [IF:7.74]

Changes in dietary fiber intake in mice reveal associations between colonic mucin O-glycosylation and specific gut bacteria

小鼠膳食纤维摄入量的变化揭示了结肠粘蛋白O-糖基化与特定肠道细菌的关系

10.1080/19490976.2020.1802209

2020-09-29, Article

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The colonic mucus layer, comprised of highly O-glycosylated mucins, is vital to mediating host-gut microbiota interactions, yet the impact of dietary changes on colonic mucin O-glycosylation and its associations with the gut microbiota remains unexplored. Here, we used an array of omics techniques including glycomics to examine the effect of dietary fiber consumption on the gut microbiota, colonic mucin O-glycosylation and host physiology of high-fat diet-fed C57BL/6J mice. The high-fat diet group had significantly impaired glucose tolerance and altered liver proteome, gut microbiota composition, and short-chain fatty acid production compared to normal chow diet group. While dietary fiber inclusion did not reverse all high fat-induced modifications, it resulted in specific changes, including an increase in the relative abundance of bacterial families with known fiber digesters and a higher propionate concentration. Conversely, colonic mucin O-glycosylation remained similar between the normal chow and high-fat diet groups, while dietary fiber intervention resulted in major alterations in O-glycosylation. Correlation network analysis revealed previously undescribed associations between specific bacteria and mucin glycan structures. For example, the relative abundance of the bacterium Parabacteroides distasonis positively correlated with glycan structures containing one terminal fucose and correlated negatively with glycans containing two terminal fucose residues or with both an N-acetylneuraminic acid and a sulfate residue. This is the first comprehensive report of the impact of dietary fiber on the colonic mucin O-glycosylation and associations of these mucosal glycans with specific gut bacteria.

First Authors:
Hasinika K A H Gamage,Raymond W W Chong,Daniel Bucio-Noble

Correspondence Authors:
Mark P Molloy,Nicolle H Packer,Ian T Paulsen

All Authors:
Hasinika K A H Gamage,Raymond W W Chong,Daniel Bucio-Noble,Liisa Kautto,Anandwardhan Hardikar,Malcolm S Ball,Mark P Molloy,Nicolle H Packer,Ian T Paulsen

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