Abstract & Authors:展开
Treatment of metastatic colorectal cancer is based upon the assumption that metastases are homogeneous within a patient. We quantified immune cell types of 603 whole-slide metastases and primary colorectal tumors from 222 patients. Primary lesions, and synchronous and metachronous metastases, had a heterogeneous immune infiltrate and mutational diversity. Small metastases had frequently a low Immunoscore and T and B cell score, while a high Immunoscore was associated with a lower number of metastases. Anti-epidermal growth factor receptor treatment modified immune gene expression and significantly increased T cell densities in the metastasis core. The predictive accuracy of the Immunoscore from a single biopsy was superior to the one of programmed cell death ligand 1 (PD-L1). The immune phenotype of the least-infiltrated metastasis had a stronger association with patient outcome than other metastases.
Marc Van den Eynde,Bernhard Mlecnik,Gabriela Bindea
Marc Van den Eynde,Bernhard Mlecnik,Gabriela Bindea,Tessa Fredriksen,Sarah E Church,Lucie Lafontaine,Nacilla Haicheur,Florence Marliot,Mihaela Angelova,Angela Vasaturo,Daniela Bruni,Anne Jouret-Mourin,Pamela Baldin,Nicolas Huyghe,Karin Haustermans,Annelies Debucquoy,Eric Van Cutsem,Jean-Francois Gigot,Catherine Hubert,Alex Kartheuser,Christophe Remue,Daniel Leonard,Viia Valge-Archer,Franck Pagès,Jean-Pascal Machiels,Jérôme Galon