王炜+李小卫+杨朝勇:新方法看清菌群在肠内的3D空间分布
  • 开发一种结合组织透明化和新型右旋氨基酸(DAA)标记的策略,能实现肠道细菌的原位3D高分辨率定量成像;
  • 首先通过腹腔注射荧光DAA探针来标记小鼠固有肠道菌群,然后使用CUBIC对肠道组织进行透明化处理,再用共聚焦显微镜进行整组织成像,从而获得肠道菌群在肠内分布情况的3D图像,并能进行定量分析;
  • 用该方法意外地在被认为是无菌的小肠隐窝中观察到菌群的存在,且在肠炎模型中检测到小肠的前三分之二肠段存在显著的细菌过度生长。
主编推荐语
mildbreeze
受限于现有的研究方法,人们对于肠道菌群在肠道内的三维空间分布情况仍所知甚少。上海交通大学的王炜、李小卫和杨朝勇与团队,在Angewandte Chemie发表的一项最新工作中,报道了一种结合了组织透明化和新型DAA在体标记策略的方法,能实现肠道细菌在肠道内的原位3D高分辨率定量成像。该方法有助于直观地揭示菌群-宿主之间的相互作用,3D荧光图像非常炫酷,推荐专业人士参考。
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Three‐dimensional Quantitative Imaging of Native Microbiota Distribution in the Gut

肠道内原生菌群分布的三维定量成像

10.1002/anie.202010921

2020-10-20, Article

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Due to the challenges to acquire detailed spatial information of gut bacteria in situ, microbiota’s three‐dimensional (3D) distributions in the gut remain largely uncharted. Here, we propose a tissue clearing‐based and D‐amino acid labeling‐facilitated (TiDaL) strategy that combines a novel microbiota in vivo labeling protocol, CUBIC‐based tissue clearing and whole‐mount tissue imaging, to achieve 3D imaging of indigenous gut microbiota. We demonstrate high resolution 3D acquisition of their biogeography in different gut sections, and present quantitative spatial details in relation to the host epithelium. We unexpectedly observe microbiota in the small intestine crypts which were thought to be bacteria‐free. Moreover, significant bacterial overgrowth in the first two‐thirds of the small intestine is detected in an enteritis model. We expect that this quantitative 3D imaging strategy for native gut microbiota will provide insightful information into the host‐microbiota interactions.

First Authors:
Wei Wang,Ni Zhang,Yahui Du

Correspondence Authors:
Wei Wang,Xiaowei Li,Chaoyong Yang

All Authors:
Wei Wang,Ni Zhang,Yahui Du,Juan Gao,Min Li,Liyuan Lin,Daniel M Czajkowsky,Xiaowei Li,Chaoyong Yang,Zhifeng Shao

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