• 180只肉鸡随机分成:活益生乳酸菌组(LJ),灭活LJ组和对照组;
  • 42天后,LJ对肉鸡平均日增重和饲料转化率有正面作用;
  • 降低肉鸡血清甘油三酯、低密度脂胆固醇水平和腹部脂肪堆积;
  • 减少脂肪组织中的脂蛋白脂肪酶和肝脏中的硬脂酰辅酶A去饱和酶-1的mRNA水平;
  • 改善肠道发育和消化能力,增加表皮生长因子、胰岛素样生长因子-1水平和空肠及回肠中的胰蛋白酶、脂肪酶活性;
  • 增加拟杆菌和乳酸杆菌数量,减少肠杆菌数,降低厚壁菌/拟杆菌比。

Live Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance and Lowers Fat Deposition by Improving Lipid Metabolism, Intestinal Development, and …

活益生乳酸菌johnsonii BS15通过改善脂质代谢、肠道发育和肠道菌群促进肉鸡生长和降低脂肪堆积


2017-06-12, Article

Abstract & Authors:展开

Numerous studies have focused on the beneficial effects of probiotics in animals. Even so, additional information should be obtained about the mechanisms by which a useful probiotic strain successfully exerts such beneficial effects. In this study, we evaluated the effect of the dietary supplementation of both live and disrupted Lactobacillus johnsonii (LJ) strain BS15 in broilers at different ages. Specifically, growth performance, lipid metabolism, gut microbiota, intestinal development, and digestive ability of the broilers were assessed. A total of 180 1-day-old Cobb 500 chicks were randomly distributed into three groups. These chicks were fed diets supplemented with 1 × 106 colony-forming units (cfu) LJ per gram of feed (LJ group); 1 × 106 cfu disrupted LJ per gram of feed (D-LJ group); and de Man, Rogosa, and Sharpe liquid medium (control group), respectively, throughout a 42-day experimental period. The results demonstrated that LJ supplementation of feed had a positive effect on the average daily gain and starter feed conversion ratio. In addition, LJ supplementation of feed decreased serum triglyceride and low-density lipid cholesterol levels, as well as abdominal fat deposition. LJ also reduced the mRNA levels of lipoprotein lipase in adipose tissue and stearoyl-CoA desaturase-1 in the liver. LJ diminished the mRNA quantities of the sterol regulatory element binding protein-1c and fatty acid synthase, as well as increased the level of serum high-density lipid cholesterol. LJ increased the mRNA quantities of peroxisome proliferator-activated receptor α, acyl-CoA oxidase in the liver, and carnitine palmitoyltransferase-1. LJ also improved the intestinal development and digestive ability mainly by increasing the villus height/crypt depth ratio in the ileum. The probiotic increased the levels of epidermal growth factor and insulin-like growth factor-1, as well as the activities of trypsin and lipase in the jejunum and ileum. LJ exerted beneficial effects on the intestinal flora. Specifically, LJ markedly enhanced the population of Bacteroidetes and Lactobacillus spp. Moreover, the probiotic reduced the population of Enterobacteriaceae and the Firmicutes/Bacteroidetes ratio. Slight changes caused by disrupted LJ were detected. These findings indicated that live LJ supplementation may promote growth performance and lower fat deposition in broilers.

First Authors:
Hesong Wang,Xueqin Ni

Correspondence Authors:
Dong Zeng

All Authors:
Hesong Wang,Xueqin Ni,Xiaodan Qing,Dong Zeng,Min Luo,Lei Liu,Guangyao Li,Kangcheng Pan,Bo Jing