多吃水果和蔬菜降低大肠癌发病的机制
  • 分别采集11名回肠造口患者食用300g覆盆子前、后的回肠液,在体外进行粪便发酵培养;
  • 发酵后回肠液中花青素等(多)酚类物质明显减少,而酚酸类和芳香族代谢产物的含量增多;
  • 9名回肠造口患者来源的覆盆子后的回肠液发酵产物显著减少DNA损伤(约30%);
  • 覆盆子回肠发酵物质调控氧化应激细胞保护的Nrf2-ARE通路的基因表达;
  • 分别检测四种酚类代谢产物的作用,发现均可在10-100μM生理相关范围内减轻DNA损伤和调节Nrf2-ARE通路关键基因。
主编推荐语
爱的抉择
富含水果和蔬菜的饮食与结直肠癌(CRC)发病率的降低有关,部分原因是生物活性(聚)酚类成分及其肠道菌群代谢物的功能。Redox Biology近期发表的文章,收集回肠造口患者食用覆盆子前、后的回肠液进行体外发酵,发现覆盆子回肠发酵物质可调控氧化应激细胞保护通路Nrf2-ARE的基因表达,显著减少DNA损伤。即结肠可利用的覆盆子(聚)酚类及其微生物衍生的分解代谢产物可能在体内对大肠癌起保护作用。
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Redox Biology [IF:9.986]

Ex vivo fecal fermentation of human ileal fluid collected after raspberry consumption modifies (poly)phenolics and modulates genoprotective effects in colonic epithelial cells

食用树莓后的人回肠液经体外粪便发酵从而调节多酚和结肠上皮细胞的基因保护作用

10.1016/j.redox.2021.101862

01-12, Article

Abstract & Authors:展开

Abstract:收起
Diets rich in fruit and vegetables are associated with a decreased incidence of colorectal cancer (CRC) due, in part, to the bioactive (poly)phenolic components and their microbiota-mediated metabolites. This study investigated how such compounds, derived from ingested raspberries in the gastrointestinal tract, may exert protective effects by reducing DNA damage. Ileal fluids collected pre- and post-consumption of 300 g of raspberries by ileostomists (n = 11) were subjected to 24 h ex vivo fermentation with fecal inoculum to simulate interaction with colonic microbiota. The impact of fermentation on (poly)phenolics in ileal fluid was determined and the bioactivity of ileal fluids pre- and post fermentation investigated. (Poly)phenolic compounds including sanguiin H-6, sanguiin H-10 and cyanidin-3-O-sophoroside decreased significantly during fermentation while, in contrast, microbial catabolites, including 3-(3′-hydroxyphenyl)propanoic acid, 3-hydroxybenzoic acid and benzoic acid increased significantly. The post-raspberry ileal fermentate from 9 of the 11 ileostomates significantly decreased DNA damage (~30%) in the CCD 841 CoN normal cell line using an oxidative challenge COMET assay. The raspberry ileal fermentates also modulated gene expression of the nuclear factor 2–antioxidant responsive element (Nrf2-ARE) pathway involved in oxidative stress cytoprotection, namely Nrf2, NAD(P)H dehydrogenase, quinone-1 and heme oxygenase-1. Four of the phenolic catabolites were assessed individually, each significantly reducing DNA damage from an oxidative challenge over a physiologically relevant 10–100 μM range. They also induced a differential pattern of expression of key genes in the Nrf2-ARE pathway in CCD 841 CoN cells. The study indicates that the colon-available raspberry (poly)phenols and their microbial-derived catabolites may play a role in protection against CRC in vivo.

First Authors:
Sara Dobani

Correspondence Authors:
Chris IR Gill

All Authors:
Sara Dobani,Cheryl Latimer,Gordon J McDougall,J William Allwood,Gema Pereira-Caro,José Manuel Moreno-Rojas,Nigel G Ternan,L Kirsty Pourshahidi,Roger Lawther,Kieran M Tuohy,Daniele Del Rio,Gloria O Connor,Ian Rowland,Tahani Mazyad Almutairi,Alan Crozier,Chris IR Gill

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