印遇龙等:褪黑素调节肠道菌群并缓解小鼠断奶压力
创作:szx 审核:szx 2018年04月08日
  • 断奶小鼠补充褪黑素后,体重增加显著提升,且肠道形态、隐窝深度、绒毛/隐窝比得到显著改善;
  • 褪黑素对肠道细胞的增殖或凋亡、Paneth细胞及杯状细胞的数量、回肠中肠上皮细胞相关标记物(蔗糖酶)及内分泌细胞相关标记物(嗜铬粒蛋白A、YY肽)表达影响较小;
  • 补充褪黑素对血清氨基酸及压力相关参数的影响较小,增加肠道菌群的丰度,并增加乳杆菌属的丰度,显著改变肠道菌群的氨基酸及药物代谢;
  • 肠道菌群可能介导了褪黑素的上述作用。
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蓝灿辉 | 热心肠先生
这是中国科学院亚热带农业生态研究所印遇龙院士领衔做的褪黑素与肠道菌群相关性研究,发现褪黑素通过调节肠道菌群缓解小鼠的断奶压力。很值得关注的研究,特别推荐。
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Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota

褪黑素缓解小鼠的断奶压力:与肠道菌群相关

10.1111/jpi.12448

2017-09-05, Article

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Melatonin influences intestinal microbiota and the pathogenesis of various diseases. This study was conducted to explore whether melatonin alleviates weanling stress through intestinal microbiota in a weanling mouse model. Melatonin supplementation in weanling mice (provided in the drinking water at a dosage of 0.2 mg/mL for 2 weeks) significantly improved body weight gain (1.4 ± 0.03 g/day in melatonin group vs 1.2 ± 0.06 g/day in control group) and intestinal morphology (ie, villus length, crypt depth, and villus to crypt ratio), but had little effect on the proliferation or apoptosis of intestinal cells, the numbers of Paneth cells and goblet cells, as well as the expression of makers related to enterocytes (sucrase) and endocrine cells (chromogranin A and peptide YY) in the ileum. Melatonin supplementation had little effect on serum levels of amino acids or stress-related parameters (eg, SOD, TNF-α, and angiotensin I). 16S rRNA sequencing suggested that melatonin supplementation increased the richness indices of intestinal microbiota (observed species, Chao 1, and ACE) and shaped the composition of intestinal microbiota (eg, increase in the abundance of Lactobacillus [19 ± 3% in melatonin group vs 6 ± 2% in control group]), which was demonstrated using an ex vivo proliferation assay and colonic loop proliferation assay. Melatonin supplementation also significantly influenced the metabolism of intestinal microbiota, such as amino acid metabolism and drug metabolism. More importantly, in antibiotic-treated weanling mice and germ-free weanling mice, melatonin failed to affect body weight gain or intestinal morphology. Melatonin significantly reduced (by about 60%) the bacterial load in enterotoxigenic Escherichia coli (ETEC)-infected weanling mice, but had little effect on ETEC load in antibiotic-pretreated animals. In conclusion, melatonin affects body weight gain, intestinal morphology, and intestinal ETEC infection through intestinal microbiota in weanling mice. The findings highlight the importance of intestinal microbiota in mediating the various physiological functions of melatonin in the host.

First Authors:
Wenkai Ren

Correspondence Authors:
Gang Liu,Bie Tan,Yulong Yin

All Authors:
Wenkai Ren,Peng Wang,Jiameng Yan,Gang Liu,Benhua Zeng,Tarique Hussain,Can Peng,Jie Yin,Tiejun Li,Hong Wei,Guoqiang Zhu,Russel J Reiter,Bie Tan,Yulong Yin

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