胃肠道类器官技术有望推动肠道病毒感染研究
创作:王辉 审核:周旸 01月06日
  • 动物模型和单细胞系在研究人类病毒中作用有限,因为感染途径和依赖的免疫系统组成都存在宿主间差异性,而单细胞系无法模拟细胞互作;
  • 类器官能更好地模拟真实的人类肠道,提供了研究人类肠道病毒感染及肠上皮细胞互作的离体平台;
  • 类器官可作为体外分离许多难培养的肠道病毒的培养模型;
  • 人胃肠道类器官可用于研究肠道病毒感染对不同细胞的倾向性,揭示受感染细胞的种类;
  • 类器官可为人体对肠道病毒感染的先天免疫反应提供新的见解。
主编推荐语
周旸
传统的动物感染、细胞感染模型,已经无法满足对于病毒感染的生物学研究,而类器官可以填补传统感染模型的技术缺陷。PLoS Pathogens近日发表评论文章,强调了类器官在肠道病毒感染研究中的多方面功能,值得参考!
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PLoS Pathogens [IF:6.218]

Gastrointestinal organoid technology advances studies of enteric virus biology

胃肠道类器官技术促进肠道病毒生物学研究

10.1371/journal.ppat.1008212

2020-01-30, Review

Abstract & Authors:展开

Abstract:收起
The historical lack of models recapitulating the complexity of the human intestinal epithelium has hindered studies into many aspects of human enteric virus biology. Immortalized and transformed cell lines are typically limited by the presence of only one cell type, whereas susceptibility in animal models often requires infection routes that differ from humans or necessitates modification of immune system components [1, 2]. In addition, comparing animals from different species remains a confounding factor when trying to infer how findings may apply to human health. Thus, the development of human gastrointestinal organoids to study virus–host interactions marks a significant advance. They provide a physiologically relevant ex vivo platform in which human enteric microorganisms can be studied interacting with the human intestinal epithelium. Furthermore, their intermediate complexity, falling between cell lines and animal models, adds an additional tool to better understand these viruses. Here, we highlight how gastrointestinal organoids have provided new insights into the biology of human enteric viruses and the potential of this technology for advancing the field.

First Authors:
Abimbola O Kolawole

Correspondence Authors:
Christiane E Wobus

All Authors:
Abimbola O Kolawole,Christiane E Wobus

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