Nature子刊:胎儿肠道中真有活菌定殖吗?
创作:mildbreeze 审核:mildbreeze 03月01日
  • 扫描电镜分析表明,孕中期胎儿小肠粘膜胎粪中存在细菌样结构;
  • 16S rRNA测序也检测出不同于环境对照的少量细菌信号,18个细菌类群在胎粪中富集,微球菌科和乳杆菌属丰度最高;
  • 微球菌科占优的胎儿肠道有独特的T细胞组成和肠上皮基因表达模式;
  • 用模拟胎儿肠道环境的培养条件(添加胎盘激素、与单核细胞共培养),从胎粪中分离出滕黄微球菌菌株;
  • 该菌呈现适应胎儿环境的特征,可在肠道抗原呈递细胞中存活,并有限制炎症反应的能力。
主编推荐语
mildbreeze
肠道菌群在免疫发育过程中有重要作用。在妊娠早期,胎儿的肠粘膜免疫就已经开始发育,然而目前尚不清楚子宫内是否存在活菌与胎儿的肠道免疫系统互作。Nature Medicine发表的一项最新研究,用扫描电子显微镜和16S rRNA测序表明,孕中期的人类胎儿肠道内存在很有限的细菌,并分离出其中的一种优势细菌(微球菌)。该菌表现出对子宫内胎儿肠道环境的适应性,并在体外呈现免疫调节活性,可能对胎儿肠道免疫发育有潜在调控作用。这些发现拓展了人们对生命早期肠道菌群定殖、菌群对免疫发育的影响的认知,值得进一步验证和研究。
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Nature Medicine [IF:30.641]

Viable bacterial colonization is highly limited in the human intestine in utero

在子宫内的人类肠道内,活细菌的定殖非常有限

10.1038/s41591-020-0761-3

02-24, Article

Abstract & Authors:展开

Abstract:收起
Mucosal immunity develops in the human fetal intestine by 11–14 weeks of gestation, yet whether viable microbes exist in utero and interact with the intestinal immune system is unknown. Bacteria-like morphology was identified in pockets of human fetal meconium at mid-gestation by scanning electron microscopy (n = 4), and a sparse bacterial signal was detected by 16S rRNA sequencing (n = 40 of 50) compared to environmental controls (n = 87). Eighteen taxa were enriched in fetal meconium, with Micrococcaceae (n = 9) and Lactobacillus (n = 6) the most abundant. Fetal intestines dominated by Micrococcaceae exhibited distinct patterns of T cell composition and epithelial transcription. Fetal Micrococcus luteus, isolated only in the presence of monocytes, grew on placental hormones, remained viable within antigen presenting cells, limited inflammation ex vivo and possessed genomic features linked with survival in the fetus. Thus, viable bacteria are highly limited in the fetal intestine at mid-gestation, although strains with immunomodulatory capacity are detected in subsets of specimens.

First Authors:
E Rackaityte

Correspondence Authors:
S V Lynch

All Authors:
E Rackaityte,J Halkias,E M Fukui,V F Mendoza,C Hayzelden,E D Crawford,K E Fujimura,T D Burt,S V Lynch

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Nature Medicine期刊

Initial exploration of in utero microbial colonization

Maria Carmen Collado, Nicola Segata,2020-03-30

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科研 | Nature Medicine:子宫内胎儿肠道内活菌定殖非常有限

仰望星空,2020-04-02

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菌探Momics微信公众号

活菌在子宫内的人类肠道定植受到高度限制

2020-03-10

点评:菌探Momics 全文解读

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