军事医学科学院毕玉晶:豚鼠肠道菌群能否改变小鼠的肺鼠疫敏感性?
  • 鼠疫耶尔森菌201菌株对小鼠有强烈毒性,但对豚鼠几乎无毒性;
  • 给小鼠喂食豚鼠饲料两月后,经口灌胃给予0.5ml的豚鼠粪便悬浮液,发现豚鼠的肠道菌群能在小鼠中定植;
  • 通过肺入侵使小鼠感染鼠疫耶尔森菌201,但定植豚鼠肠道菌群的小鼠和对照小鼠的存活率,未发现统计学差异;
  • 移植来自豚鼠的肠道菌群,不影响小鼠对肺鼠疫的敏感性,其肠道菌群对小鼠肺鼠疫无防御作用。
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鼠疫耶尔森菌201菌株可在小鼠中致病,但对豚鼠几乎无毒性,给小鼠定殖豚鼠的肠道菌群,能否减轻该菌对小鼠的感染性?PeerJ近期发表来自军事医学科学院团队的研究,通过菌群移植实验,对这一问题进行了解答。
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PeerJ [IF:2.379]

Successful transplantation of guinea pig gut microbiota in mice and its effect on pneumonic plague sensitivity

小鼠成功移植豚鼠肠道菌群及其对肺鼠疫敏感性的影响

10.7717/peerj.5637

2018-09-24, Article

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Microbiota-driven variations in the inflammatory response are predicted to regulate host responses to infection. Increasing evidence indicates that the gastrointestinal and respiratory tracts have an intimate relationship with each other. Gut microbiota can influence lung immunity whereby gut-derived injurious factors can reach the lungs and systemic circulation via the intestinal lymphatics. The intestinal microbiota’s ability to resist colonization can be extended to systemic infections or to pathogens infecting distant sites such as the lungs. Unlike the situation with large mammals, the microtus Yersinia pestis 201 strain exhibits strong virulence in mice, but nearly no virulence to large mammals (such as guinea pigs). Hence, to assess whether the intestinal microbiota from guinea pigs was able to affect the sensitivity of mice to challenge infection with the Y. pestis 201 strain, we fed mice with guinea pig diets for two months, after which they were administered 0.5 ml of guinea pig fecal suspension for 30 days by oral gavage. The stools from each mouse were collected on days 0, 15, and 30, DNA was extracted from them, and 16S rRNA sequencing was performed to assess the diversity and composition of the gut microbiota. We found that the intestinal microbiota transplants from the guinea pigs were able to colonize the mouse intestines. The mice were then infected with Yersinia pestis 201 by lung invasion, but no statistical difference was found in the survival rates of the mice that were colonized with the guinea pig’s gut microbiota and the control mice. This indicates that the intestinal microbiota transplantation from the guinea pigs did not affect the sensitivity of the mice to pneumonic plague.

First Authors:
Xiang Li

Correspondence Authors:
Yujing Bi

All Authors:
Xiang Li,Zhengchao Li,Yuxiao Chang,Fengyi Hou,Zongyu Huang,Han Ni,Ruifu Yang,Yujing Bi

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