• 为研究人类肠道菌群及其代谢产物和一天的时间、饮食行为之间的关系,对来自28名健康男女的77份粪便样本进行分析;
  • 一天中,乙酸、丙酸和丁酸的浓度随时间降低;
  • 35%细菌OTU与时间有关,肠道菌群与进食频率、早间能量消耗和夜间禁食时间长短有关;
  • 肠道菌群的组成和功能随一天时间的推移而变化,可能与人昼夜节律、微生物群落本身或饮食行为有关;
  • 进食时间和夜间禁食时长可预测菌群丰度,但需纵向干预研究来确定其因果关系。
蓝灿辉 | 热心肠先生

Time of day and eating behaviors are associated with the composition and function of the human gastrointestinal microbiota



2017-09-27, Article

Abstract & Authors:展开

Background: Preclinical research has shown that the gastrointestinal microbiota exhibits circadian rhythms and that the timing of food consumption can affect the composition and function of gut microbes. However, there is a dearth of knowledge on these relations in humans.Objective: We aimed to determine whether human gastrointestinal microbes and bacterial metabolites were associated with time of day or behavioral factors, including eating frequency, percentage of energy consumed early in the day, and overnight-fast duration.Design: We analyzed 77 fecal samples collected from 28 healthy men and women. Fecal DNA was extracted and sequenced to determine the relative abundances of bacterial operational taxonomic units (OTUs). Gas chromatography-mass spectroscopy was used to assess short-chain fatty acid concentrations. Eating frequency, percentage of energy consumed before 1400, and overnight-fast duration were determined from dietary records. Data were analyzed by linear mixed models or generalized linear mixed models, which controlled for fiber intake, sex, age, body mass index, and repeated sampling within each participant. Each OTU and metabolite were tested as the outcome in a separate model.Results: Acetate, propionate, and butyrate concentrations decreased throughout the day (P = 0.006, 0.04, and 0.002, respectively). Thirty-five percent of bacterial OTUs were associated with time. In addition, relations were observed between gut microbes and eating behaviors, including eating frequency, early energy consumption, and overnight-fast duration.Conclusions: These results indicate that the human gastrointestinal microbiota composition and function vary throughout the day, which may be related to the circadian biology of the human body, the microbial community itself, or human eating behaviors. Behavioral factors, including timing of eating and overnight-fast duration, were also predictive of bacterial abundances. Longitudinal intervention studies are needed to determine causality of these biological and behavioral relations. This trial was registered at clinicaltrials.gov as NCT01925560.

First Authors:
Jennifer L Kaczmarek

Correspondence Authors:
Hannah D Holscher

All Authors:
Jennifer L Kaczmarek,Salma Ma Musaad,Hannah D Holscher