Nature:婴儿肠道病毒组定植分两步,母乳喂养保护作用显著
  • 婴儿肠道病毒组的定植和组装可分为2个阶段;
  • 胎粪和早期粪便样本中基本不存在病毒样颗粒(VLP),但1月龄时每克粪便中的VLP数量增长至10亿(与成人接近),主要是早期定植的“先锋”细菌所诱导的原噬菌体(即细菌自带的温和噬菌体);
  • 至4月龄时,婴儿肠道中具有感染性的病毒开始增多,该阶段的病毒和噬菌体组成主要受喂养方式影响,母乳喂养对多种病毒有明显的抑制作用,并能增加双歧杆菌属和乳杆菌属的温和噬菌体。
主编推荐语
mildbreeze
建立正常的肠道菌群对于婴儿的健康发育有重要意义,目前相关研究大多聚焦肠道细菌,人们对婴儿肠道病毒组还所知有限。Nature发表的一项最新研究,分析了20名婴儿出生、1月龄和4月龄时的粪便病毒组及其影响因素,并在另外2个超过百人的婴儿队列中进行验证。该研究发现,婴儿肠道病毒组的定植可分为2个阶段:早期以先锋细菌诱导的温和噬菌体为主,之后逐渐出现可感染人体细胞的病毒,而这些病毒在母乳喂养的婴儿中明显较少。这项研究不仅揭示了生命早期肠道病毒组的发展规律,也为母乳喂养的积极作用提供了新的见解。
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Nature [IF:42.778]

The stepwise assembly of the neonatal virome is modulated by breastfeeding

新生儿病毒组的分步组装是通过母乳喂养调节的

10.1038/s41586-020-2192-1

2020-04-15, Article

Abstract & Authors:展开

Abstract:收起
The gut of healthy human neonates is usually devoid of viruses at birth, but quickly becomes colonized, which—in some cases—leads to gastrointestinal disorders1,2,3,4. Here we show that the assembly of the viral community in neonates takes place in distinct steps. Fluorescent staining of virus-like particles purified from infant meconium or early stool samples shows few or no particles, but by one month of life particle numbers increase to 109 per gram, and these numbers seem to persist throughout life5,6,7. We investigated the origin of these viral populations using shotgun metagenomic sequencing of virus-enriched preparations and whole microbial communities, followed by targeted microbiological analyses. Results indicate that, early after birth, pioneer bacteria colonize the infant gut and by one month prophages induced from these bacteria provide the predominant population of virus-like particles. By four months of life, identifiable viruses that replicate in human cells become more prominent. Multiple human viruses were more abundant in stool samples from babies who were exclusively fed on formula milk compared with those fed partially or fully on breast milk, paralleling reports that breast milk can be protective against viral infections8,9,10. Bacteriophage populations also differed depending on whether or not the infant was breastfed. We show that the colonization of the infant gut is stepwise, first mainly by temperate bacteriophages induced from pioneer bacteria, and later by viruses that replicate in human cells; this second phase is modulated by breastfeeding.

First Authors:
Guanxiang Liang

Correspondence Authors:
Frederic D Bushman

All Authors:
Guanxiang Liang,Chunyu Zhao,Huanjia Zhang,Lisa Mattei,Scott Sherrill-Mix,Kyle Bittinger,Lyanna R Kessler,Gary D Wu,Robert N Baldassano,Patricia DeRusso,Eileen Ford,Michal A Elovitz,Matthew S Kelly,Mohamed Z Patel,Tiny Mazhani,Jeffrey S Gerber,Andrea Kelly,Babette S Zemel,Frederic D Bushman

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Medical Xpress新闻网站

Breastfeeding may lead to fewer human viruses in infants

2020-04-15

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转化医学网微信公众号

【Nature】母乳喂养或有助于预防胃肠道病毒感染

转网,2020-04-16

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生物探索微信公众号

Nature:母乳喂养可以调节新生儿肠道病毒定植?

王煊,2020-04-20

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Nature Reviews Microbiology期刊

Prophages are gut virome pioneers

2020-04-21

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