吸烟如何影响十二指肠粘膜菌群
创作:szx 审核:szx 2018年09月04日
  • 选取102名受试者进行内窥镜检查,其中有21名吸烟者、40名曾是吸烟者、41名非吸烟者(从未吸烟),分析十二指肠菌群;
  • 与非吸烟者相比,吸烟者(无论是现在吸烟还是过去吸烟)与十二指肠粘膜菌群的细菌多样性显著降低相关;
  • 吸烟者的十二直肠菌群中厚壁菌门(特别是链球菌属及韦荣球菌属)的丰度升高,而普氏菌属及奈瑟氏菌属的丰度降低;
  • 过去吸烟的受试者的十二直肠菌群谱介于现仍在吸烟的受试者与非吸烟者之间。
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szx
吸烟是胃肠道疾病的风险因素之一,但其对上消化道粘膜相关菌群的影响研究较少。Microbiome上发表的一项最新研究发现,吸烟(无论是否已戒烟)显著影响了十二指肠粘膜菌群的多样性及组成。
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Microbiome [IF:11.607]

Influence of cigarette smoking on the human duodenal mucosa-associated microbiota

吸烟对人十二指肠粘膜相关菌群的影响

10.1186/s40168-018-0531-3

2018-08-29, Article

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BACKGROUND: Cigarette smoking is a known risk factor in a number of gastrointestinal (GI) diseases in which the microbiota is implicated, including duodenal ulcer and Crohn's disease. Smoking has the potential to alter the microbiota; however, to date, the impact of smoking on the mucosa-associated microbiota (MAM), and particularly that of the upper GI tract, remains very poorly characterised. Thus, we investigated the impact of smoking on the upper small intestinal MAM. A total of 102 patients undergoing upper GI endoscopy for the assessment of GI symptoms, iron deficiency, or Crohn's disease, but without identifiable lesions in the duodenum, were recruited. Smoking status was determined during clinical assessment and patients classified as current (n = 21), previous smokers (n = 40), or having never smoked (n = 41). The duodenal (D2) MAM was profiled via 16S rRNA gene amplicon sequencing.
RESULTS: Smoking, both current and previous, is associated with significantly reduced bacterial diversity in the upper small intestinal mucosa, as compared to patients who had never smoked. This was accompanied by higher relative abundance of Firmicutes, specifically Streptococcus and Veillonella spp. The relative abundance of the genus Rothia was also observed to be greater in current smokers; while in contrast, levels of Prevotella and Neisseria were lower. The MAM profiles and diversity of previous smokers were observed to be intermediate between current and never smokers. Smoking did not impact the total density of bacteria present on the mucosa.
CONCLUSIONS: These data indicate the duodenal MAM of current smokers is characterised by reduced bacterial diversity, which is partially but not completely restored in previous smokers. While the precise mechanisms remain to be elucidated, these microbiota changes may in some part explain the adverse effects of smoking on mucosa-associated diseases of the GI tract. Smoking status requires consideration when interpreting MAM data.

First Authors:
Erin R Shanahan

Correspondence Authors:
Mark Morrison,Gerald J Holtmann

All Authors:
Erin R Shanahan,Ayesha Shah,Natasha Koloski,Marjorie M Walker,Nicholas J Talley,Mark Morrison,Gerald J Holtmann

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