• 成年雄性大鼠,喂链霉素/青霉素,行淋巴瘘管手术,经一夜恢复,十二指肠注射脂肪乳剂;
  • 肠道淋巴粘膜肥大细胞蛋白酶II减少,二胺氧化酶活性下降,脂质渗透性下降,三酰甘油和磷脂转运减少,粘膜载脂蛋白B、AI和A4水平下降,肠蠕动及胰脂肪酶活性则无差异;
  • 急性给予抗生素或停药后4周,上述效应消失;
  • 证明摄入脂肪后,肠道菌群激活肥大细胞,促进脂质渗透性,菌群还可能通过肠道产生载脂蛋白和分泌乳糜微粒,促进脂类吸收。
蓝灿辉 | 热心肠先生
Gastroenterology [IF:17.373]

Antibiotics Suppress Activation of Intestinal Mucosal Mast Cells and Reduce Dietary Lipid Absorption in Sprague-Dawley Rats



2016-07-16, Article

Abstract & Authors:展开

BACKGROUND & AIMS: The gut microbiota affects intestinal permeability and mucosal mast cell (MMCs) responses. Activation of MMCs has been associated with absorption of dietary fat. We investigated whether the gut microbiota contributes to the fat-induced activation of MMCs in rats, and how antibiotics might affect this process.
METHODS: Adult male Sprague-Dawley rats were given streptomycin and penicillin for 4 days (n=6-8), to reduce the abundance of their gut flora, or normal drinking water (controls, n=6-8). They underwent lymph fistula surgery and after an overnight recovery were given an intraduodenal bolus of intralipid. We collected intestinal tissues and lymph fluid and assessed activation of MMCs, intestinal permeability, and fat transport parameters.
RESULTS: Compared with controls, intestinal lymph from rats given antibiotics had reduced levels of mucosal mast cell protease II (produced by MMCs) and decreased activity of diamine oxidase (produced by enterocytes) (P<.05). Rats given antibiotics had reduced intestinal permeability in response to dietary lipid, compared with controls (P<.01). Unexpectedly, antibiotics also reduced lymphatic transport of triacylglycerol and phospholipid (P<.01), concomitant with decreased levels of mucosal apolipoproteins B, AI, and A4 (P<.01). No differences were found in intestinal motility or luminal pancreatic lipase activity between rats given antibiotics and controls. These effects were not seen with an acute dose of antibiotics or 4 weeks after the antibiotic regimen ended.
CONCLUSIONS: The intestinal microbiota appears to activate MMCs following the ingestion of fat in rats-this contributes to fat-induced intestinal permeability. We found that the gut microbiome promotes absorption of lipid, probably by intestinal production of apolipoproteins and secretion of chylomicrons.

First Authors:
Hirokazu Sato

Correspondence Authors:
Patrick Tso

All Authors:
Hirokazu Sato,Linda Zhang,Kristina Martinez,Eugene B Chang,Qing Yang,Fei Wang,Philip N Howles,Ryota Hokari,Soichiro Miura,Patrick Tso