酷炫:肿瘤相关巨噬细胞3D成像!
  • 肿瘤进展过程中免疫系统的参与,是肿瘤研究的最前沿;
  • 在小鼠肺癌模型中,采用组织光透明技术以及注射肿瘤相关巨噬细胞(TAM)靶向荧光纳米颗粒(NP),可进行快速肿瘤体积分析,获得在全肺内细胞水平上TAM浸润的空间信息;
  • TAM的密度,在相同动物上,不同肿瘤之间,具有异质性,独立的肺肿瘤模型中,TAM总密度不同,纳米药物输送与TAM异质性相关;
  • 提高TAM在全部肿瘤和肿瘤内的穿透力,可改善集落刺激因子1受体(CSF-1R)阻断剂的疗效。
关键字
延伸阅读本研究的原文信息和链接出处,以及相关解读和评论文章。欢迎读者朋友们推荐!
图片

Heterogeneity of macrophage infiltration and therapeutic response in lung carcinoma revealed by 3D organ imaging

3D器官成像技术揭示肺癌中巨噬细胞浸润及治疗疗效的异质性

10.1038/ncomms14293

2017-02-08, Article

Abstract & Authors:展开

Abstract:收起
Involvement of the immune system in tumour progression is at the forefront of cancer research. Analysis of the tumour immune microenvironment has yielded a wealth of information on tumour biology, and alterations in some immune subtypes, such as tumour-associated macrophages (TAM), can be strong prognostic indicators. Here, we use optical tissue clearing and a TAM-targeting injectable fluorescent nanoparticle (NP) to examine three-dimensional TAM composition, tumour-to-tumour heterogeneity, response to colony-stimulating factor 1 receptor (CSF-1R) blockade and nanoparticle-based drug delivery in murine pulmonary carcinoma. The method allows for rapid tumour volume assessment and spatial information on TAM infiltration at the cellular level in entire lungs. This method reveals that TAM density was heterogeneous across tumours in the same animal, overall TAM density is different among separate pulmonary tumour models, nanotherapeutic drug delivery correlated with TAM heterogeneity, and successful response to CSF-1R blockade is characterized by enhanced TAM penetration throughout and within tumours.

First Authors:
Michael F Cuccarese

Correspondence Authors:
Mikael J Pittet,Ralph Weissleder

All Authors:
Michael F Cuccarese,J Matthew Dubach,Christina Pfirschke,Camilla Engblom,Christopher Garris,Miles A Miller,Mikael J Pittet,Ralph Weissleder

评论