• 检测在胃癌不同组织学阶段中、及接受幽门螺旋杆菌(HP)清除治疗后患者的胃部菌群;
  • 取HP胃炎、胃粘膜的肠上皮化生、胃癌患者及HP阴性对照者的内窥镜胃活组织样本,并分析胃部菌群;
  • 包括嗜血杆菌属、沙雷氏菌属、奈瑟氏菌属及寡养单胞菌的其它变形菌门成员是胃部菌群的主要成分;
  • HP相对丰度与细菌多样性之间有着强烈的负相关性,这种相关性在胃癌样本中较弱,相比于有着相似HP水平的其他受试者,胃癌患者的胃部菌群细菌多样性较低。
蓝灿辉 | 热心肠先生
Scientific Reports [IF:3.998]

Alterations in Gastric Microbiota After H. Pylori Eradication and in Different Histological Stages of Gastric Carcinogenesis



2017-03-21, Article

Abstract & Authors:展开

The role of bacteria other than Helicobacter pylori (HP) in the stomach remains elusive. We characterized the gastric microbiota in individuals with different histological stages of gastric carcinogenesis and after receiving HP eradication therapy. Endoscopic gastric biopsies were obtained from subjects with HP gastritis, gastric intestinal metaplasia (IM), gastric cancer (GC) and HP negative controls. Gastric microbiota was characterized by Illumina MiSeq platform targeting the 16 S rDNA. Apart from dominant H. pylori, we observed other Proteobacteria including Haemophilus, Serratia, Neisseria and Stenotrophomonas as the major components of the human gastric microbiota. Although samples were largely converged according to the relative abundance of HP, a clear separation of GC and other samples was recovered. Whilst there was a strong inverse association between HP relative abundance and bacterial diversity, this association was weak in GC samples which tended to have lower bacterial diversity compared with other samples with similar HP levels. Eradication of HP resulted in an increase in bacterial diversity and restoration of the relative abundance of other bacteria to levels similar to individuals without HP. In conclusion, HP colonization results in alterations of gastric microbiota and reduction in bacterial diversity, which could be restored by antibiotic treatment.

First Authors:
Tung Hiu Li

Correspondence Authors:
Wai K Leung

All Authors:
Tung Hiu Li,Youwen Qin,Pak Chung Sham,K S Lau,Kent-Man Chu,Wai K Leung