衰老小鼠的菌群可诱导肥胖
创作:szx 审核:szx 2020年10月21日
  • 收集成年小鼠(100-300日龄)及衰老小鼠(550-750日龄)的粪便与血液样本进行对比分析;
  • 衰老小鼠与成年小鼠的粪便菌群有显著差异,衰老小鼠的厚壁菌门/拟杆菌门比值更高,萨特氏菌属等5个菌属的相对丰度升高;
  • 衰老小鼠的体重及脂肪量更高,而瘦体重更低,且血液中的胰岛素及瘦素水平更高;
  • 将衰老小鼠的粪菌移植给成年小鼠,后者表现出肥胖相关表型:脂肪量及胰岛素水平更高,摄入的食物更多,且有着更高的呼吸商。
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Genome Medicine上发表的一项最新研究,对比鉴定了衰老小鼠与成年小鼠的粪便菌群及血液代谢指标差异,将衰老小鼠的粪菌移植给成年小鼠,可诱导后者表现出肥胖相关表型,包括更高的脂肪量、胰岛素水平、呼吸商及食物摄入,提示衰老小鼠的肠道菌群具有致肥胖性特征。
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Genome Medicine [IF:10.675]

The aging mouse microbiome has obesogenic characteristics

衰老小鼠菌群具有致肥胖性特征

10.1186/s13073-020-00784-9

2020-10-12, Article

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BACKGROUND: During aging, there is a physiological decline, an increase of morbidity and mortality, and a natural change in the gut microbiome. In this study, we investigated the influence of the gut microbiome on different metabolic parameters in adult and aged mice.
METHODS: Fecal and blood samples from adult (n = 42, 100-300 days) and aging (n = 32, 550-750 days) mice were collected. Microbiome analysis was done using QIIME2. Mouse weight and body composition were measured using NMR, and insulin and leptin levels in the blood were measured with Mouse Adipokine Magnetic Bead Panel kit. Fecal microbiota transplantation experiments from adult and aged mice into young germ-free mice were carried out in order to examine the effect of the gut microbiome of adult and aging mice on weight, body composition, insulin, and leptin.
RESULTS: We demonstrate that the microbiomes from adult and aged mice are distinguishable. We also report changes in metabolic parameters as we observed significantly higher weight and fat mass and low lean mass in aged compared to adult mice along with high insulin and leptin levels in the blood. The transplanted gut microbiome from aged mice transferred part of the phenotypes seen in aged mice. Fat body mass and insulin levels were higher in the mice who received feces from aged mice than mice receiving feces from adult mice. In addition, they consumed more food and had a higher respiratory quotient compared to mice receiving adult feces.
CONCLUSIONS: We conclude that aged mice have a gut microbiota with obesogenic characteristics. In addition, the gut bacterial population itself is sufficient to induce some of the manifestations of obesity.

First Authors:
Dana Binyamin,Nir Werbner

Correspondence Authors:
Omry Koren

All Authors:
Dana Binyamin,Nir Werbner,Meital Nuriel-Ohayon,Atara Uzan,Hadar Mor,Atallah Abbas,Oren Ziv,Raffaele Teperino,Roee Gutman,Omry Koren

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