Abstract & Authors:展开
Objectives: Gut microbiome alterations in Parkinson's disease (PD) have been reported repeatedly, but their functional relevance remains unclear. Fecal metabolomics, which provide a functional readout of microbial activity, have scarcely been investigated. We investigated fecal microbiome and metabolome alterations in PD, and their clinical relevance.
Methods: Two‐hundred subjects (104 patients, 96 controls) underwent extensive clinical phenotyping. Stool samples were analysed using 16S rRNA gene sequencing. Fecal metabolomics were performed using two platforms, nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography‐mass spectrometry.
Results: Fecal microbiome and metabolome composition in PD were significantly different vs. controls, with the largest effect size seen in NMR‐based metabolome. Microbiome and NMR‐based metabolome compositional differences remained significant after comprehensive confounder analyses. Differentially abundant fecal metabolite features and predicted functional changes in PD vs. controls included bioactive molecules with putative neuroprotective effects (e.g., short chain fatty acids [SCFAs], ubiquinones, and salicylate), and other compounds increasingly implicated in neurodegeneration (e.g., ceramides, sphingosine, and trimethylamine N‐oxide). In the PD group, cognitive impairment, low body mass index (BMI), frailty, constipation, and low physical activity were associated with fecal metabolome compositional differences. Notably, low SCFAs in PD were significantly associated with poorer cognition and low BMI. Lower butyrate levels correlated with worse postural instability‐gait disorder scores.
Interpretation: Gut microbial function is altered in PD, characterized by differentially abundant metabolic features that provide important biological insights into gut‐brain pathophysiology. Their clinical relevance further supports a role for microbial‐metabolites as potential targets for the development of new biomarkers and therapies in PD.
Ai Huey Tan
Ai Huey Tan,Chun Wie Chong
Ai Huey Tan,Chun Wie Chong,Shen-Yang Lim,Ivan Kok Seng Yap,Cindy Shuan,Ju Teh,Mun Fai Loke,Sze-Looi Song,Jiun Yan Tan,Ban Hong Ang,Yong Qi Tan,Mee Teck Kho,Jeff Bowman,Sanjiv Mahadeva,Hoi Sen Yong,Anthony E Lang