仝小林、王军等:葛根芩连汤调菌群降血糖,小檗碱是关键成分
创作:szx 审核:szx 2020年12月30日
  • 在糖尿病大鼠模型中,GQD及其组分小檗碱有着相当的降血糖作用,并均可使肠道菌群结构恢复至与正常大鼠相似;
  • GQD及小檗碱处理均可导致粪杆菌属、罗斯氏菌属等丁酸盐产生菌的富集,并增加粪便中的短链脂肪酸水平;
  • GQD及小檗碱可相似地调控回肠基因的表达,下调免疫应答相关基因,上调脂代谢相关基因;
  • 另外,GQD及小檗碱均可降低血清中的促炎细胞因子浓度,并减少胰岛中的炎症相关基因的表达,以缓解系统性及局部炎症。
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szx
葛根芩连汤(GQD)是一种常用于2型糖尿病的中药配方。来自中科院微生物所的王军团队、广安门医院的仝小林院士和赵林华与团队,在Genomics, Proteomics and Bioinformatics上发表的一项最新研究,发现GQD可通过其关键组分——小檗碱调节肠道菌群的组成,富集丁酸盐产生菌,调控回肠基因表达,并降低全身及胰岛的炎症因子表达,从而降低糖尿病大鼠的血糖。
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Antidiabetic Effects of Gegen Qinlian Decoction via the Gut Microbiota Are Attributable to Its Key Ingredient Berberine

葛根芩连汤通过肠道菌群发挥抗糖尿病作用的关键成分是小檗碱

10.1016/j.gpb.2019.09.007

2020-12-21, Article

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Gegen Qinlian Decoction (GQD), a traditional Chinese medicine (TCM) formula, has long been used for the treatment of common metabolic diseases, including type 2 diabetes mellitus. However, the main limitation of its wider application is ingredient complexity of this formula. Thus, it is critically important to identify the major active ingredients of GQD and to illustrate mechanisms underlying its action. Here, we compared the effects of GQD and berberine, a hypothetical key active pharmaceutical ingredient of GQD, on a diabetic rat model by comprehensive analyses of gut microbiota, short-chain fatty acids, proinflammatory cytokines, and ileum transcriptomics. Our results show that berberine and GQD had similar effects on lowering blood glucose levels, modulating gut microbiota, inducing ileal gene expression, as well as relieving systemic and local inflammation. As expected, both berberine and GQD treatment significantly altered the overall gut microbiota structure and enriched many butyrate-producing bacteria, including Faecalibacterium and Roseburia, thereby attenuating intestinal inflammation and lowering glucose. Levels of short-chain fatty acids in rat feces were also significantly elevated after treatment with berberine or GQD. Moreover, concentration of serum proinflammatory cytokines and expression of immune-related genes, including Nfkb1, Stat1, and Ifnrg1, in pancreatic islets were significantly reduced after treatment. Our study demonstrates that the main effects of GQD can be attributed to berberine via modulating gut microbiota. The strategy employed would facilitate further standardization and widespread application of TCM in many diseases.

First Authors:
Xizhan Xu,Zezheng Gao

Correspondence Authors:
Linhua Zhao,Xiaolin Tong,Jun Wang

All Authors:
Xizhan Xu,Zezheng Gao,Fuquan Yang,Yingying Yang,Liang Chen,Lin Han,Na Zhao,Jiayue Xu,Xinmiao Wang,Yue Ma,Lian Shu,Xiaoxi Hu,Na Lyu,Yuanlong Pan,Baoli Zhu,Linhua Zhao,Xiaolin Tong,Jun Wang

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