对213株乳杆菌及其相关菌属进行比较基因组学研究扩展乳酸杆菌的生物应用潜力
  • 乳酸菌的代谢多样性、种属不明确、与其他重要的乳酸菌之间关系的不确定性,使得开发利用乳酸菌变得复杂,且乳酸菌催化的生物转化能力尚未开发;
  • 发布213株乳杆菌及其相关菌属的基因组序列,发现73个核心基因,描述修饰碳水化合物和蛋白质的编码基因目录;
  • 乳酸杆菌有广泛且多样性的新CRISPR-Cas免疫系统,可用于基因编辑;
  • 揭示乳酸杆菌在自然和工业环境的宿主相互作用因子和细菌素的系统基因组学分布,及工艺过程中承受压力的机制。
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Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera

10.1038/ncomms9322

2015-09-29, Article

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Lactobacilli are a diverse group of species that occupy diverse nutrient-rich niches associated with humans, animals, plants and food. They are used widely in biotechnology and food preservation, and are being explored as therapeutics. Exploiting lactobacilli has been complicated by metabolic diversity, unclear species identity and uncertain relationships between them and other commercially important lactic acid bacteria. The capacity for biotransformations catalysed by lactobacilli is an untapped biotechnology resource. Here we report the genome sequences of 213 Lactobacillus strains and associated genera, and their encoded genetic catalogue for modifying carbohydrates and proteins. In addition, we describe broad and diverse presence of novel CRISPR-Cas immune systems in lactobacilli that may be exploited for genome editing. We rationalize the phylogenomic distribution of host interaction factors and bacteriocins that affect their natural and industrial environments, and mechanisms to withstand stress during technological processes. We present a robust phylogenomic framework of existing species and for classifying new species.

First Authors:
Zhihong Sun,Hugh M B Harris,Angela McCann,Chenyi Guo,Silvia Argimón,Wenyi Zhang

Correspondence Authors:
Yujun Cui,Heping Zhang,Paul W O'Toole

All Authors:
Zhihong Sun,Hugh M B Harris,Angela McCann,Chenyi Guo,Silvia Argimón,Wenyi Zhang,Xianwei Yang,Ian B Jeffery,Jakki C Cooney,Todd F Kagawa,Wenjun Liu,Yuqin Song,Elisa Salvetti,Agnieszka Wrobel,Pia Rasinkangas,Julian Parkhill,Mary C Rea,Orla O'Sullivan,Jarmo Ritari,François P Douillard,R Paul Ross,Ruifu Yang,Alexandra E Briner,Giovanna E Felis,Willem M De Vos,Rodolphe Barrangou,Todd R Klaenhammer,Page W Caufield,Yujun Cui,Heping Zhang,Paul W O'Toole

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