• 对比分析胃贲门癌患者的80份胃部组织样本(非恶性组织及恶性肿瘤组织)的菌群组成及结构,用PICRUSt预测菌群功能;
  • 与无上消化道癌症家庭疾病史的患者的胃部菌群相比,有家庭疾病史的患者胃部非恶性组织菌群中幽门螺旋杆菌(HP)相对丰度更高,α多样性更低;
  • 有着更高肿瘤分级的患者胃部非恶性组织菌群中HP相对丰度更高,α多样性更低,5个KEGG功能性模块相对丰度发生显著改变;
  • 无转移的患者胃部非恶性组织菌群中乳酸杆菌丰度更高。
蓝灿辉 | 热心肠先生

Gastric microbiota features associated with cancer risk factors and clinical outcomes: A pilot study in gastric cardia cancer patients from Shanxi, China



2017-03-20, Article

Abstract & Authors:展开

Little is known about the link between gastric microbiota and the epidemiology of gastric cancer. In order to determine the epidemiologic and clinical relevance of gastric microbiota, we used 16S ribosomal RNA gene sequencing analysis to characterize the composition and structure of the gastric microbial community of 80 paired samples (non-malignant and matched tumor tissues) from gastric cardia adenocarcinoma (GCA) patients in Shanxi, China. We also used PICRUSt to predict microbial functional profiles. Compared to patients without family history of upper gastrointestinal (UGI) cancer in the non-malignant gastric tissue microbiota, patients with family history of UGI cancer had higher Helicobacter pylori (Hp) relative abundance (median: 0.83 vs. 0.38, P=0.01) and lower alpha diversity (median observed species: 51 vs. 85, P=0.01). Patients with higher (versus lower) tumor grade had higher Hp relative abundance (0.73 vs. 0.18, P=0.03), lower alpha diversity (observed species, 66 vs. 89, p=0.01), altered beta diversity (weighted UniFrac, P=0.002), and significant alterations in relative abundance of five KEGG functional modules in non-malignant gastric tissue microbiota. Patients without metastases had higher relative abundance of Lactobacillales than patients with metastases (0.05 vs. 0.01, P=0.04) in non-malignant gastric tissue microbiota. These associations were observed in non-malignant tissues but not in tumor tissues. In conclusion, this study showed a link of gastric microbiota to a major gastric cancer risk factor and clinical features in GCA patients from Shanxi, China. Studies with both healthy controls and gastric cardia and noncardia cancer cases across different populations are needed to further examine the association between gastric cancer and the microbiota. This article is protected by copyright. All rights reserved.

First Authors:
Guoqin Yu

Correspondence Authors:
Guoqin Yu,Alisa M Goldstein

All Authors:
Guoqin Yu,Nan Hu,Lemin Wang,Chaoyu Wang,Xiao-You Han,Mike Humphry,Jacques Ravel,Christian C Abnet,Philip R Taylor,Alisa M Goldstein