Science封面文章:巧用工程菌,调控蜜蜂基因表达、限制病原体感染
创作:mildbreeze 审核:mildbreeze 03月01日
  • 将靶向特定基因的双链RNA(dsRNA)质粒,转化到蜜蜂肠道共生菌Snodgrassella alvi中,使其能经RNA干扰(RNAi)机制调控宿主基因表达;
  • 这种工程菌能在蜜蜂肠道中稳定定植,持续产生激活蜜蜂RNAi的dsRNA,dsRNA能扩散到蜜蜂全身从而系统性抑制特定蜜蜂基因的表达,改变蜜蜂生理、行为和生长;
  • 靶向残翅病毒的工程菌可增强蜜蜂对其感染的抵抗力,靶向蜜蜂寄生虫瓦螨的工程菌可通过触发瓦螨的RNAi应答来将其杀死。
主编推荐语
mildbreeze
真核生物中,RNA干扰(RNAi)能引起基因沉默,是研究基因功能的有力手段,在传染病治疗和某些疾病的基因治疗方面也有应用前景,Science最新发表的封面研究将RNAi与蜜蜂的肠道共生菌进行了有机结合,使得改造后的工程菌能在蜜蜂肠道中持续释放特定的双链RNA,从而激活蜜蜂的RNAi应答,可用于研究蜜蜂基因功能。此外,这种工程菌方法还能减少特定病原体(比如病毒和寄生螨虫)对蜜蜂的感染,或许有助于改善蜜蜂养殖。
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Science [IF:41.845]

Engineered symbionts activate honey bee immunity and limit pathogens

工程共生菌激活蜜蜂免疫力并限制病原体

10.1126/science.aax9039

01-31, Report

Abstract & Authors:展开

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Honey bees are essential pollinators threatened by colony losses linked to the spread of parasites and pathogens. Here, we report a new approach for manipulating bee gene expression and protecting bee health. We engineered a symbiotic bee gut bacterium, Snodgrassella alvi, to induce eukaryotic RNA interference (RNAi) immune responses. We show that engineered S. alvi can stably recolonize bees and produce double-stranded RNA to activate RNAi and repress host gene expression, thereby altering bee physiology, behavior, and growth. We used this approach to improve bee survival after a viral challenge, and we show that engineered S. alvi can kill parasitic Varroa mites by triggering the mite RNAi response. This symbiont-mediated RNAi approach is a tool for studying bee functional genomics and potentially for safeguarding bee health.

First Authors:
Sean P Leonard

Correspondence Authors:
Jeffrey E Barrick,Nancy A Moran

All Authors:
Sean P Leonard,J Elijah Powell,Jiri Perutka,Peng Geng,Luke C Heckmann,Richard D Horak,Bryan W Davies,Andrew D Ellington,Jeffrey E Barrick,Nancy A Moran

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2020-02-17

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Modified bugs boost bee health

2020-02-19

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