中国医学科学院:动物模型揭示SARS-CoV-2感染胃肠道的证据
创作:EADGBE 审核:EADGBE 2020年12月23日
  • 使用恒河猴模型,探究鼻内和腹腔接种SARS-CoV-2病毒对宿主的影响;
  • 鼻内接种SARS-CoV-2后,可同时在呼吸道和消化道组织检测到病毒RNA;
  • 病毒鼻内接种诱导炎症细胞因子的表达上调,导致呼吸道和消化道病理损伤;
  • 腹腔接种SARS-CoV-2可导致肺炎和呼吸道损伤,并上调炎症细胞因子的表达水平;
  • 腹腔接种病毒后,可在肠系膜淋巴、血液、胰腺和肝组织及粪便中检测到病毒RNA;
  • 腹腔接种SARS-CoV-2可在感染早期损伤肠道屏障功能。
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EADGBE
2019年冠状病毒肺炎(COVID-19)患者显示出越来越多的胃肠道症状。然而,胃肠道在SARS-CoV-2感染中的作用尚不完全清楚。来自中国医学科学院北京协和医学院的彭小忠、刘红旗和鲁帅尧等人发表在Gastroenterology上的一项以恒河猴为对象的研究表明,鼻内和胃内接种新型冠状病毒SARS-CoV-2可引起肺炎和胃肠功能障碍,而炎症细胞因子可能是COVID-19中呼吸系统和消化系统病变之间的联系,该研究成果也为新冠病毒的粪口传播提供了有利证据,值得参考。
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Gastroenterology [IF:22.682]

The gastrointestinal tract is an alternative route for SARS-CoV-2 infection in a nonhuman primate model

胃肠道是非人灵长类动物SARS-CoV-2感染的替代途径

10.1053/j.gastro.2020.12.001

2020-12-08, Article

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Background and Aims: Gastrointestinal (GI) manifestations have been increasingly reported in Coronavirus Disease 2019 (COVID-19) patients. However, the roles of the GI tract in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection are not fully understood. We investigated how the GI tract is involved in SARS-CoV-2 infection to elucidate the pathogenesis of COVID-19.
Methods: Our previously established nonhuman primate (NHP) model of COVID-19 was modified in this study to test our hypothesis. Rhesus monkeys were infected with an intragastric or intranasal challenge with SARS-CoV-2. Clinical signs were recorded after infection. Viral genomic RNA was quantified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Host responses to SARS-CoV-2 infection were evaluated by examining inflammatory cytokines, macrophages, histopathology and mucin barrier integrity.
Results: Intranasal inoculation with SARS-CoV-2 led to infections and pathological changes not only in respiratory tissues but also in digestive tissues. Expectedly, intragastric inoculation with SARS-CoV-2 resulted in the productive infection of digestive tissues and inflammation in both the lung and digestive tissues. Inflammatory cytokines were induced by both types of inoculation with SARS-CoV-2, consistent with the increased expression of CD68. Immunohistochemistry and alcian blue/periodic acid-Schiff (AB-PAS) staining showed decreased Ki67, increased cleaved caspase 3 and decreased numbers of mucin-containing goblet cells, suggesting that the inflammation induced by these two types of inoculation with SARS-CoV-2 impaired the GI barrier and caused severe infections.
Conclusions: Both intranasal and intragastric inoculation with SARS-CoV-2 caused pneumonia and GI dysfunction in our rhesus monkey model. Inflammatory cytokines are possible connections for the pathogenesis of SARS-CoV-2 between the respiratory and digestive systems.

First Authors:
Li Jiao,Haiyan Li,Jingwen Xu,Mengli Yang

Correspondence Authors:
Shuaiyao Lu,Hongqi Liu,Xiaozhong Peng

All Authors:
Li Jiao,Haiyan Li,Jingwen Xu,Mengli Yang,Chunxia Ma,Jingmei Li,Siwen Zhao,Haixuan Wang,Yun Yang,Wenhai Yu,Junbin Wang,Jing Yang,Haiting Long,Jiahong Gao,Kaiyun Ding,Daoju Wu,Dexuan Kuang,Yuan Zhao,Jiansheng Liu,Shuaiyao Lu,Hongqi Liu,Xiaozhong Peng

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