Cell子刊:胃峡干细胞是胃腺上皮更新的主力军
  • 胃体腺可分为两个独立的区域,基底部由慢更新的基底干细胞维持,凹-峡-颈部(基底部以上区域)由快速更新的峡部干细胞(IsthSC)维持;
  • 用细胞增殖标志物Stmn1和Ki67进行谱系追踪,证实了IsthSC的作用和特性;
  • IsthSC在胃腺中的克隆扩张速度在纵向上较快,而在横向上因峡部壁细胞的限制而呈现“间断”式的慢速扩张;
  • 单细胞RNA测序鉴定出IsthSC的基因表达特征,包括高表达Stmn1和Ki67、低表达分化标志物Muc5ac和Muc6。
主编推荐语
mildbreeze
胃腺是胃粘膜上皮凹陷形成的腺体结构,从下往上可分为基底部、颈部、峡部和凹部四个区域。与肠隐窝底部的肠道干细胞不同,胃腺基底部的干细胞在大多数时间都处于静息状态,仅在上皮损伤时才被激活,因此必然存在其它干细胞来维持胃腺和胃上皮的快速细胞更新。有研究认为,胃腺的峡部也存在干细胞,但对于这种干细胞,人们所知有限。Cell Stem Cell近期发表的一项研究,通过多种试验手段对小鼠的胃峡干细胞的特性进行了深入分析。
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Cell Stem Cell [IF:20.86]

Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells

定义成年胃峡部干细胞的特性和动态

10.1016/j.stem.2019.07.008

2019-08-15, Article

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Abstract:收起
The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of “punctuated” neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.

First Authors:
Seungmin Han,Juergen Fink

Correspondence Authors:
Jong Kyoung Kim,Benjamin D Simons,Bon-Kyoung Koo

All Authors:
Seungmin Han,Juergen Fink,David J Jorg,Eunmin Lee,Min Kyu Yum,Lemonia Chatzeli,Sebastian R Merker,Manon Josserand,Teodora Trendafilova,Amanda Andersson-Rolf,Catherine Dabrowska,Hyunki Kim,Ronald Naumann,Ji-Hyun Lee,Nobuo Sasaki,Richard Lester Mort,Onur Basak,Hans Clevers,Daniel E Stange,Anna Philpott,Jong Kyoung Kim,Benjamin D Simons,Bon-Kyoung Koo

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