• 纳入783名有1型糖尿病(T1D)遗传风险的白人儿童,分析粪便宏基因组的纵向变化,并进行巢式病例对照分析;
  • 对照儿童菌群中,与细菌发酵和短链脂肪酸生物合成相关的基因含量更高,但未鉴定出具有普遍性的与胰岛自身免疫或 T1D显著相关的菌群成员;
  • 婴儿肠道菌群发育呈现动态变化和个体差异,出生第一年内以双歧杆菌属(两歧/短/长双歧杆菌)或变形菌门为优势菌;
  • 含有利用人乳寡糖基因的长双歧杆菌菌株,特异存在于母乳喂养婴儿中。
Nature [IF:42.778]

The human gut microbiome in early-onset type 1 diabetes from the TEDDY study



2018-10-24, Article

Abstract & Authors:展开

Type 1 diabetes (T1D) is an autoimmune disease that targets pancreatic islet beta cells and incorporates genetic and environmental factors, including complex genetic elements, patient exposures and the gut microbiome. Viral infections and broader gut dysbioses have been identified as potential causes or contributing factors; however, human studies have not yet identified microbial compositional or functional triggers that are predictive of islet autoimmunity or T1D. Here we analyse 10,913 metagenomes in stool samples from 783 mostly white, non-Hispanic children. The samples were collected monthly from three months of age until the clinical end point (islet autoimmunity or T1D) in the The Environmental Determinants of Diabetes in the Young (TEDDY) study, to characterize the natural history of the early gut microbiome in connection to islet autoimmunity, T1D diagnosis, and other common early life events such as antibiotic treatments and probiotics. The microbiomes of control children contained more genes that were related to fermentation and the biosynthesis of short-chain fatty acids, but these were not consistently associated with particular taxa across geographically diverse clinical centres, suggesting that microbial factors associated with T1D are taxonomically diffuse but functionally more coherent. When we investigated the broader establishment and development of the infant microbiome, both taxonomic and functional profiles were dynamic and highly individualized, and dominated in the first year of life by one of three largely exclusive Bifidobacterium species (B. bifidum, B. breve or B. longum) or by the phylum Proteobacteria. In particular, the strain-specific carriage of genes for the utilization of human milk oligosaccharide within a subset of B. longum was present specifically in breast-fed infants. These analyses of TEDDY gut metagenomes provide, to our knowledge, the largest and most detailed longitudinal functional profile of the developing gut microbiome in relation to islet autoimmunity, T1D and other early childhood events. Together with existing evidence from human cohorts and a T1D mouse model, these data support the protective effects of short-chain fatty acids in early-onset human T1D.

First Authors:
Tommi Vatanen

Correspondence Authors:
Curtis Huttenhower,Ramnik J Xavier

All Authors:
Tommi Vatanen,Eric A Franzosa,Randall Schwager,Surya Tripathi,Timothy D Arthur,Kendra Vehik,Åke Lernmark,William A Hagopian,Marian J Rewers,Jin-Xiong She,Jorma Toppari,Anette-G Ziegler,Beena Akolkar,Jeffrey P Krischer,Christopher J Stewart,Nadim J Ajami,Joseph F Petrosino,Dirk Gevers,Harri Lähdesmäki,Hera Vlamakis,Curtis Huttenhower,Ramnik J Xavier


Nature | TEDDY研究中早发1型糖尿病的肠道微生物组


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