复旦肿瘤医院:用游离癌细胞培养类器官,提供抗癌新方法
创作:周旸 审核:周旸 01月06日
  • 采用过滤和CD45耗竭技术,从26名诊断为转移型结直肠癌患者的血液中分离出随血液循环的肿瘤细胞(CTC);
  • 经过14天培养得到初代CTC类器官,再次接种后得到多个体积较小的次代CTC类器官;
  • 在CTC类器官中可以鉴别出两种体积不一致的CTC细胞,均具有巨大的细胞核结构和细胞质结构;
  • 相较于从患者肿瘤组织细胞培养得到的类器官,CTC类器官具有形态特异性。
主编推荐语
周旸
无法通过手术方法治疗的转移型结直肠癌,对治疗提出了新挑战。复旦大学附属肿瘤医院团队近期在Clinical and Translational Medicine发表通讯文章,介绍了通过血液游离癌细胞培养类器官的方法,为建立转移型结直肠癌的个体化治疗方法提供了一个研究平台,值得参考。
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Establishment and identification of organoids from human circulating colorectal cancer cells

利用人体血液中的结直肠癌细胞构建与鉴定类器官

10.1002/ctm2.247

11-08, Other

Abstract & Authors:展开

Abstract:收起
Patients diagnosed with metastatic colorectal cancer (mCRC) often suffer from unresectable metastases, which lead to a rather poor prognosis.1 Although efforts have been made, the need for personalized therapy for mCRC patients is still urgent. Human CRC organoids derived from CRC patients’ primary tumor samples closely recapitulate several properties of the original tumor and provide a platform for personalized therapy design.2,3 However, the dependence of resected primary samples of PDO model limited its application in mCRC patients. Circulating tumor cells (CTCs) are tumor cells that have detached from the primary tumor and circulate in the peripheral blood of patients, which are thought to be critical for cancer metastasis.4 Thus, CTCs have been approved and are used in clinical trials as an important component of liquid biopsies for metastatic breast, prostate, and colorectal cancers.5 It has been reported that CTC organoids were established from patients with advanced prostate cancer,6 providing evidence for the feasibility of culturing organoids from CRC CTCs, and this inspired us to establish a CTC organoid model for future personalized therapy exploration in mCRC patients suffering from unresectable metastases.

First Authors:
Long Zhang,Shaobo Mo,Xiang Hu

Correspondence Authors:
Sanjun Cai,Yaqi Li,Junjie Peng

All Authors:
Long Zhang,Shaobo Mo,Xiang Hu,Xiaoji Ma,Weixiang Jin,David Haixiang Peng,Yu Zhou,Xiaofei Liang,Xinxin Rao,Xiaoya Xu,Zhen Zhang,Guoqiang Hua,Sanjun Cai,Yaqi Li,Junjie Peng

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