Science子刊;TMPRSS2与TMPRSS4促进新冠病毒感染肠上皮细胞
创作:aluba 审核:aluba 2020年06月02日
  • SARS-CoV-2可感染人小肠类器官,并在ACE2+ 人成熟肠上皮细胞中复制;
  • 两种粘膜特异性丝氨酸蛋白酶——TMPRSS2与TMPRSS4可促进SARS-CoV-2进入肠上皮细胞以感染小肠类器官;
  • 在人胃肠道中,因胃液的低pH值及结肠液中的某些成分,SARS-CoV-2快速失活而失去了感染能力;
  • 收集10份COVID-19患者的粪便样本,在其中3份中可检测到SARS-CoV-2病毒基因组的高RNA拷贝数,但无法分离出有感染性的病毒。
主编推荐语
aluba
Science Immunology上发表的一项最新研究,利用小肠类器官模型研究了SARS-CoV-2的感染机制,发现两种高表达于人肠上皮细胞中的丝氨酸蛋白酶——TMPRSS2与TMPRSS4,可促进SARS-CoV-2对ACE2+ 肠上皮细胞的感染。另外,当SARS-CoV-2被释放至肠腔中时,结肠液可导致SARS-CoV-2失活而失去感染能力。
关键字
延伸阅读本研究的原文信息和链接出处,以及相关解读和评论文章。欢迎读者朋友们推荐!
图片
Science Immunology [IF:30.63]

TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes

TMPRSS2与TMPRSS4促进SARS-CoV-2对人小肠肠上皮细胞的感染

10.1126/sciimmunol.abc3582

2020-05-13, Article

Abstract & Authors:展开

Abstract:收起
Gastrointestinal symptoms and fecal shedding of SARS-CoV-2 RNA are frequently observed in COVID-19 patients. However, it is unclear whether SARS-CoV-2 replicates in the human intestine and contributes to possible fecal-oral transmission. Here, we report productive infection of SARS-CoV-2 in ACE2+ mature enterocytes in human small intestinal enteroids. Expression of two mucosa-specific serine proteases, TMPRSS2 and TMPRSS4, facilitated SARS-CoV-2 spike fusogenic activity and promoted virus entry into host cells. We also demonstrate that viruses released into the intestinal lumen were inactivated by simulated human colonic fluid, and infectious virus was not recovered from the stool specimens of COVID-19 patients. Our results highlight the intestine as a potential site of SARS-CoV-2 replication, which may contribute to local and systemic illness and overall disease progression.

First Authors:
Ruochen Zang,Maria Florencia Gomez Castro

Correspondence Authors:
Siyuan Ding

All Authors:
Ruochen Zang,Maria Florencia Gomez Castro,Broc T McCune,Qiru Zeng,Paul W Rothlauf,Naomi M Sonnek,Zhuoming Liu,Kevin F Brulois,Xin Wang,Harry B Greenberg,Michael S Diamond,Matthew A Ciorba,Sean P J Whelan,Siyuan Ding

评论