Cell:肠道共生菌如何活化蔬菜中的营养素硫代葡萄糖苷
创作:mildbreeze 审核:mildbreeze 03月01日
  • 肠道菌群可将蔬菜中的硫代葡萄糖苷(GS)转化为有潜在健康益处的异硫氰酸酯(ITC);
  • 从多形拟杆菌(Bt)中鉴定出将GS转化ITC的关键操纵子(BT2159-BT2156,编码4个酶);
  • 在脆弱拟杆菌中表达该操纵子,可使其获得代谢GS的能力;
  • 体外实验表明,Bt代谢GS产生ITC需要BT2158以及BT2156或BT2157;
  • 给喂食GS的小鼠单一定植敲除BT2157的Bt突变菌株,会导致肠道内的ITC生成减少;
  • 该操纵子在多个人群的肠道菌群中普遍存在,并具有转录活性。
主编推荐语
mildbreeze
有研究显示,摄入西兰花、菜花、卷心菜等蔬菜中富含的营养物质硫代葡萄糖苷(GS),与某些癌症风险的降低相关。其中,肠道菌群代谢GS产生的小分子物质异硫氰酸酯(ITC),被认为具有潜在的抗癌作用。Cell最新发表来自美国斯坦福大学Elizabeth Sattely团队的研究,揭示了肠道菌群中的多形拟杆菌将GS转化为ITC的遗传和生化机制,为研究肠道菌如何代谢膳食营养素提供了一个范例。
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Cell [IF:38.637]

A Metabolic Pathway for Activation of Dietary Glucosinolates by a Human Gut Symbiont

人肠道共生菌活化膳食硫代葡萄糖苷的代谢通路

10.1016/j.cell.2020.01.023

02-20, Article

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Consumption of glucosinolates, pro-drug-like metabolites abundant in Brassica vegetables, has been associated with decreased risk of certain cancers. Gut microbiota have the ability to metabolize glucosinolates, generating chemopreventive isothiocyanates. Here, we identify a genetic and biochemical basis for activation of glucosinolates to isothiocyanates by Bacteroides thetaiotaomicron, a prominent gut commensal species. Using a genome-wide transposon insertion screen, we identified an operon required for glucosinolate metabolism in B. thetaiotaomicron. Expression of BT2159-BT2156 in a non-metabolizing relative, Bacteroides fragilis, resulted in gain of glucosinolate metabolism. We show that isothiocyanate formation requires the action of BT2158 and either BT2156 or BT2157 in vitro. Monocolonization of mice with mutant BtΔ2157 showed reduced isothiocyanate production in the gastrointestinal tract. These data provide insight into the mechanisms by which a common gut bacterium processes an important dietary nutrient.

First Authors:
Catherine S Liou,Shannon J Sirk

Correspondence Authors:
Elizabeth S Sattely

All Authors:
Catherine S Liou,Shannon J Sirk,Camil AC Diaz,Andrew P Klein,Curt R Fischer,Steven K Higginbottom,Amir Erez,Mohamed S Donia,Justin L Sonnenburg,Elizabeth S Sattely

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Nature Reviews Gastroenterology and Hepatology期刊

Brassica vegetable metabolism by gut microbiota

2020-03-11

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