段丽萍团队:小檗碱或能调节菌群,改善绝经后焦虑
  • 建立去卵巢大鼠(OVX)的实验性焦虑模型,用小檗碱(BBR)干预4周;
  • BBR可显著改善OVX大鼠的焦虑样行为;
  • BBR降低了肠道菌群丰富度和多样性,但使拟杆菌、双歧杆菌、乳杆菌和阿克曼氏菌等有益菌属丰度升高,增加菌群代谢产物雌马酚(有潜在的类雌激素作用)的生成;
  • BBR对OVX无菌大鼠没有显著的抗焦虑作用,而无菌大鼠在粪菌移植后重现了供体的焦虑症状和BBR的治疗效果;
  • 肠道菌群在BBR治疗卵巢激素缺乏所致焦虑中有重要作用。
主编推荐语
mildbreeze
小檗碱可调节肠道菌群从而发挥多种改善健康的作用,但其对绝经后情绪障碍的影响尚不清楚。北京大学第三医院段丽萍团队近期在Pharmacological Research发表研究,在大鼠模型中,首次发现小檗碱可通过调节肠道菌群,促进菌群代谢产生雌马酚,改善由雌激素缺乏引发的焦虑样行为,为治疗绝经后情绪障碍提供了新思路。
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Berberine ameliorates ovariectomy-induced anxiety-like behaviors by enrichment in equol generating gut microbiota

小檗碱通过富集产生雌马酚的肠道微生物群,改善卵巢切除诱导的类焦虑行为

10.1016/j.phrs.2021.105439

01-22, Article

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The gut microbiota is recognized as a promising therapeutic target for anxiety. Berberine (BBR) has shown efficacy in the treatment of diseases such as postmenopausal osteoporosis, obesity, and type 2 diabetes through regulating the gut microbiota. However, the effects of BBR on postmenopausal anxiety are still unclear. The purpose of the study is to test whether BBR ameliorates anxiety by modulating intestinal microbiota under estrogen-deficient conditions. Experimental anxiety was established in specific pathogen-free (SPF) ovariectomized (OVX) rats, which were then treated with BBR for 4 weeks before undergoing behavioral tests. Open field and elevated plus maze tests demonstrated that BBR treatment significantly ameliorated anxiety-like behaviors of OVX rats compared with vehicle-treated counterparts. Moreover, as demonstrated by 16S rRNA sequencing and liquid chromatography/mass spectrometry (LC/MS) analysis, BBR-treated OVX rats harbored a higher abundance of beneficial gut microbes, such as Bacteroides, Bifidobacterium, Lactobacillus, and Akkermansia, and exhibited increased equol generation. Notably, gavage feeding of BBR had no significant anti-anxiety effects on germ-free (GF) rats that underwent ovariectomy, whereas GF rats transplanted with fecal microbiota from SPF rats substantially phenocopied the donor rats in terms of anxiety-like symptoms and isoflavone levels. This study indicates that the gut microbiota is critical in the treatment of ovariectomy-aggravated anxiety, and that BBR modulation of the gut microbiota is a promising therapeutic strategy for treating postmenopausal symptoms of anxiety.

First Authors:
Yuan Fang

Correspondence Authors:
Liping Duan

All Authors:
Yuan Fang,Jindong Zhang,Shiwei Zhu,Meibo He,Shurong Ma,Qiong Jia,Qinghua Sun,Lijin Song,Yan Wang,Liping Duan

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