肉芽肿性多血管炎患者的鼻菌群变化
创作:szx 审核:szx 2019年11月16日
  • 纳入12名活动性肉芽肿性多血管炎(aGPA)患者,44名非活动性(inGPA)患者、13名疾病对照(3名显微镜下多血管炎、10名嗜酸细胞GPA)、4名家庭健康对照(aPGA患者的家庭成员)、11名健康对照;
  • 相比于inGPA患者,aGPA患者的鼻菌群中检测到金黄色葡萄球菌的比例更高;
  • 相比于健康对照,aGPA患者的鼻菌群中金黄色葡萄球菌的丰度增加,而表皮葡萄球菌的丰度降低;
  • GPA患者鼻菌群的分支酸合成及维生素B12通路的相关基因丰度显著升高。
主编推荐语
szx
先前研究发现,肉芽肿性多血管炎患者的鼻菌群中金黄色葡萄球菌增加,且后者的定殖与疾病复发风险增加相关。来自Microbiome上发表的一项横断面研究,分析鉴定出了肉芽肿性多血管炎患者的鼻菌群组成及功能的变化,发现活动性肉芽肿性多血管炎患者的鼻菌群以金黄色葡萄球菌增加及表皮葡萄球菌减少为特征。
关键字
延伸阅读本研究的原文信息和链接出处,以及相关解读和评论文章。欢迎读者朋友们推荐!
图片
Microbiome [IF:10.465]

The composition and functional protein subsystems of the human nasal microbiome in granulomatosis with polyangiitis: a pilot study

肉芽肿性多血管炎患者的鼻菌群组成与功能蛋白

10.1186/s40168-019-0753-z

2019-10-22, Article

Abstract & Authors:展开

Abstract:收起
BACKGROUND: Ear, nose and throat involvement in granulomatosis with polyangiitis (GPA) is frequently the initial disease manifestation. Previous investigations have observed a higher prevalence of Staphylococcus aureus in patients with GPA, and chronic nasal carriage has been linked with an increased risk of disease relapse. In this cross-sectional study, we investigated changes in the nasal microbiota including a detailed analysis of Staphylococcus spp. by shotgun metagenomics in patients with active and inactive granulomatosis with polyangiitis (GPA). Shotgun metagenomic sequence data were also used to identify protein-encoding genes within the SEED database, and the abundance of proteins then correlated with the presence of bacterial species on an annotated heatmap.
RESULTS: The presence of S. aureus in the nose as assessed by culture was more frequently detected in patients with active GPA (66.7%) compared with inactive GPA (34.1%). Beta diversity analysis of nasal microbiota by bacterial 16S rRNA profiling revealed a different composition between GPA patients and healthy controls (P = 0.039). Beta diversity analysis of shotgun metagenomic sequence data for Staphylococcus spp. revealed a different composition between active GPA patients and healthy controls and disease controls (P = 0.0007 and P = 0.0023, respectively), and between healthy controls and inactive GPA patients and household controls (P = 0.0168 and P = 0.0168, respectively). Patients with active GPA had a higher abundance of S. aureus, mirroring the culture data, while healthy controls had a higher abundance of S. epidermidis. Staphylococcus pseudintermedius, generally assumed to be a pathogen of cats and dogs, showed an abundance of 13% among the Staphylococcus spp. in our cohort. During long-term follow-up of patients with inactive GPA at baseline, a higher S. aureus abundance was not associated with an increased relapse risk. Functional analyses identified ten SEED protein subsystems that differed between the groups. Most significant associations were related to chorismate synthesis and involved in the vitamin B12 pathway.
CONCLUSION: Our data revealed a distinct dysbiosis of the nasal microbiota in GPA patients compared with disease and healthy controls. Metagenomic sequencing demonstrated that this dysbiosis in active GPA patients is manifested by increased abundance of S. aureus and a depletion of S. epidermidis, further demonstrating the antagonist relationships between these species. SEED functional protein subsystem analysis identified an association between the unique bacterial nasal microbiota clusters seen mainly in GPA patients and an elevated abundance of genes associated with chorismate synthesis and vitamin B12 pathways. Further studies are required to further elucidate the relationship between the biosynthesis genes and the associated bacterial species.

First Authors:
Josef Wagner,Ewan M Harrison,Marcos Martinez Del Pero

Correspondence Authors:
Andreas Kronbichler

All Authors:
Josef Wagner,Ewan M Harrison,Marcos Martinez Del Pero,Beth Blane,Gert Mayer,Johannes Leierer,Seerapani Gopaluni,Mark A Holmes,Julian Parkhill,Sharon J Peacock,David R W Jayne,Andreas Kronbichler

评论