浙工大团队:抗生素破坏肠道菌群、脂质代谢和炎症反应
  • 青霉素(Pen G)及红霉素(Ery)在全球范围内被过度使用;
  • 本研究表明,Pen、Ery、及两者混合物长期暴露导致C57小鼠肠道菌群紊乱及脂质代谢失调,且该菌群失调需要近10周禁药才能恢复;
  • 其中药物暴露使小鼠肠内拟杆菌门(主要为革兰氏阴性菌)丰度显著降低,肠道内LPS产量增加,导致更多的LPS进入血液,诱发小鼠肝脏产生低水平炎症;
  • 而肝脏的低水平炎症可能进一步导致小鼠肝脏脂质代谢紊乱。
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蓝灿辉 | 热心肠先生
浙江工业大学团队的研究,发现青霉素(Pen G)及红霉素(Ery)使用后,小鼠肠道菌群、脂质代谢和炎症反应都发生破坏,具体是什么情况,可以读读全文。
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Exposure to Oral Antibiotics Induces Gut Microbiota Dysbiosis Associated with Lipid Metabolism Dysfunction and Low-Grade Inflammation in Mice

抗生素扰乱的肠道菌群对小鼠脂质代谢及部分器官炎症反应的影响

10.1093/toxsci/kfw150

2016-08-07, Article

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BACKGROUND: Due to a long history of improper and excessive use, Penicillin G (Pen G) and erythromycin (Ery) are regularly detected in environmental samples and pose a great threat to human health.
METHODS: Here, we set out to investigate effects of Pen G, Ery or their mixture on lipid metabolism and gut microbiota in order to better understand their toxicological mechanisms. Male C57BL/6J mice were exposed either to 60 μg/ml Pen G, Ery or a half mixture of both for 6 weeks or to 10 μg/ml Pen G, Ery or a half mixture of both for 14 weeks. In a recovery experiment, male mice were exposed to 60 μg/ml Pen G or Ery for 2 weeks and then maintained without antibiotics for up to 8 weeks.
RESULTS: It was observed that oral exposure to Pen G, Ery or their mixture induced lipid metabolism dysfunction, characterized by significantly increased lipid accumulations, triglycerides (TG) levels and expression of key genes involved in free fatty acid (FFA) synthesis, FFA transport and TG synthesis in the liver. In addition, Pen G and Ery exposure induced an inflammatory response as indicated by the increase of serum lipopolysaccharide levels and the up-regulation of key genes that regulate immune responses in the liver, fat, colon and ileum. Moreover, Pen G and Ery exposure rapidly and dramatically altered the composition of the microbiota in feces and cecum. Furthermore, high throughput sequencing of V3-V4 region of bacterial 16S rRNA gene revealed additional significant changes in the cecal microbiota of antibiotics-treated mice. Importantly, it took a very long time to reconstitute the normal composition of the gut microbiota after it was imbalanced by antibiotics exposure.
CONCLUSION: Orally administered Pen G and Ery (especially to the latter) can induce gut microbiota dysbiosis, which may indirectly link antibiotic exposure to host metabolic disorders and inflammation.

First Authors:
Yuanxiang Jin

Correspondence Authors:
Zhengwei Fu

All Authors:
Yuanxiang Jin,Yan Wu,Zhaoyang Zeng,Cuiyuan Jin,Sisheng Wu,Yueyi Wang,Zhengwei Fu

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