华南农大:真菌毒素诱导肝脏炎症,肠道菌群是帮凶
创作:EADGBE 审核:兵兵 2019年12月05日
  • 口服赭曲霉毒素A(OTA)导致鸭盲肠微生物丰度和多样性降低;
  • OTA通过增加盲肠透性,增加肝脏中拟杆菌丰度和LPS含量;
  • 抗生素处理后,鸭肠道拟杆菌丰度急剧降低,OTA对其肠道微生物组成影响甚微,且无法引起肝脏炎症;
  • 移植OTA处理的鸭粪便至抗生素处理鸭后,使后者拟杆菌丰度和LPS含量增加并诱导肝脏炎症;
  • 肠道菌群,尤其是拟杆菌,参与影响OTA诱发的肝脏炎症,为霉菌中毒的预防和治疗开辟了新窗口。
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兵兵
华南农业大学杨琳和任文凯团队。赭曲毒素A(OTA)是常见的真菌毒素,主要靶点是肝脏和肾脏,鸭子对其最敏感。本研究利用OTA诱导鸭子,并监测鸭子的肝脏和肠道菌群的变化。研究结果显示,OTA诱导后,鸭子的肝脏中出现拟杆菌,且LPS含量升高。文章推测,OTA导致鸭子的肠道通透性改变,从而使菌群得以进入肝脏。随后,文章分别采用抗生素处理和粪菌移植的方法,炎症了肠道菌群在OTA诱导肝脏炎症中的作用。
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Microbiome [IF:11.607]

Ochratoxin A induces liver inflammation: involvement of intestinal microbiota

赭曲霉毒素A诱导肝脏炎症:肠道菌群的参与

10.1186/s40168-019-0761-z

2019-11-28, Article

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Background: Ochratoxin A (OTA) is a widespread mycotoxin and induces liver inflammation to human and various species of animals. The intestinal microbiota has critical importance in liver inflammation; however, it remains to know whether intestinal microbiota mediates the liver inflammation induced by OTA. Here, we treated ducklings with oral gavage of OTA (235 μg/kg body weight) for 2 weeks. Then, the microbiota in the cecum and liver were analyzed with 16S rRNA sequencing, and the inflammation in the liver was analyzed. To explore the role of intestinal microbiota in OTA-induced liver inflammation, intestinal microbiota was cleared with antibiotics and fecal microbiota transplantation was conducted.
Results: Here, we find that OTA treatment in ducks altered the intestinal microbiota composition and structure [e.g., increasing the relative abundance of lipopolysaccharides (LPS)-producing Bacteroides], and induced the accumulation of LPS and inflammation in the liver. Intriguingly, in antibiotic-treated ducks, OTA failed to induce these alterations in the liver. Notably, with the fecal microbiota transplantation (FMT) program, in which ducks were colonized with intestinal microbiota from control or OTA-treated ducks, we elucidated the involvement of intestinal microbiota, especially Bacteroides, in liver inflammation induced by OTA.
Conclusions: These results highlight the role of gut microbiota in OTA-induced liver inflammation and open a new window for novel preventative or therapeutic intervention for mycotoxicosis.

First Authors:
Wence Wang,Shuangshuang Zhai,Yaoyao Xia

Correspondence Authors:
Wenkai Ren,Lin Yang

All Authors:
Wence Wang,Shuangshuang Zhai,Yaoyao Xia,Hao Wang,Dong Ruan,Ting Zhou,Yongwen Zhu,Hongfu Zhang,Minhong Zhang,Hui Ye,Wenkai Ren,Lin Yang

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