魏泓+谢鹏等:肠道菌群经海马体中糖皮质激素受体途径影响小鼠行为
创作:奇奇 审核:周旸 2018年09月20日
  • 与SPF小鼠相比,无菌小鼠表现出较强的抗焦虑和抗抑郁行为, 大脑海马体中糖皮质激素受体通路相关的6个基因表达上调,血清皮质醇水平无显著差异;
  • 摄取大肠杆菌脂多糖后,SPF小鼠表现出抗抑郁行为,但无抗焦虑行为,上述6个相关基因中的Stat5a和Ampd3表达上调,血清皮质醇水平下降;
  • 无菌小鼠移植了抑郁病人的粪菌后,表现出抑郁和焦虑行为,Stat5a基因表达下调,血清皮质醇水平与移植健康人粪菌的小鼠没有显著差异。
主编推荐语
周旸
肠道菌群对神经系统有重要影响。魏泓和谢鹏团队利用小鼠模型,证明肠道菌群的有无、粪便菌群的来源对宿主的焦虑、抑郁行为有显著影响,该影响的作用机制与大脑海马体中糖皮质激素受体通路有关,对研究脑肠轴、菌群-宿主互作具有参考价值。
关键字
延伸阅读本研究的原文信息和链接出处,以及相关解读和评论文章。欢迎读者朋友们推荐!

Gut microbiota regulates mouse behaviors through glucocorticoid receptor pathway genes in the hippocampus

肠道菌群通过影响海马体中糖皮质激素受体途径影响小鼠行为

10.1038/s41398-018-0240-5

2018-09-07, Article

Abstract & Authors:展开

Abstract:收起
Gut microbiota has an important role in the immune system, metabolism, and digestion, and has a significant effect on the nervous system. Recent studies have revealed that abnormal gut microbiota induces abnormal behaviors, which may be associated with the hypothalamic-pituitary-adrenal (HPA) axis. Therefore, we investigated the behavioral changes in germ-free (GF) mice by behavioral tests, quantified the basal serum cortisol levels, and examined glucocorticoid receptor pathway genes in hippocampus using microarray analysis followed by real-time PCR validation, to explore the molecular mechanisms by which the gut microbiota influences the host's behaviors and brain function. Moreover, we quantified the basal serum cortisol levels and validated the differential genes in an Escherichia coli-derived lipopolysaccharide (LPS) treatment mouse model and fecal "depression microbiota" transplantation mouse model by real-time PCR. We found that GF mice showed antianxiety- and antidepressant-like behaviors, whereas E. coli LPS-treated mice showed antidepressant-like behavior, but did not show antianxiety-like behavior. However, "depression microbiota" recipient mice exhibited anxiety- and depressive-like behaviors. In addition, six glucocorticoid receptor pathway genes (Slc22a5, Aqp1, Stat5a, Ampd3, Plekhf1, and Cyb561) were upregulated in GF mice, and of these only two (Stat5a and Ampd3) were upregulated in LPS-treated mice, whereas the shared gene, Stat5a, was downregulated in "depression microbiota" recipient mice. Furthermore, basal serum cortisol levels were decreased in E. coli LPS-treated mice but not in GF mice and "depression microbiota" recipient mice. These results indicated that the gut microbiota may lead to behavioral abnormalities in mice through the downstream pathway of the glucocorticoid receptor. Herein, we proposed a new insight into the molecular mechanisms by which gut microbiota influence depressive-like behavior.

First Authors:
Yuanyuan Luo,Benhua Zeng,Li Zeng,Xiangyu Du

Correspondence Authors:
Hong Wei,Peng Xie

All Authors:
Yuanyuan Luo,Benhua Zeng,Li Zeng,Xiangyu Du,Bo Li,Ran Huo,Lanxiang Liu,Haiyang Wang,Meixue Dong,Junxi Pan,Peng Zheng,Chanjuan Zhou,Hong Wei,Peng Xie

评论