iPSC衍生的肠道模型,将在药物研发中发挥大作用(综述)
创作:女巫 审核:mildbreeze 2018年07月09日
  • 药物早期研发阶段,基于细胞的肠道模型是评估药物吸收和代谢谱的重要工具;
  • 但现有模型所用的细胞系,在药物转运蛋白和代谢酶的表达方面,与人肠细胞相比存在差异;
  • 诱导多能干细胞(iPSC)可通过重编程使成人细胞达到多能状态,而无胚胎干细胞相关的伦理顾虑;
  • iPSC可分化为肠上皮样细胞,形成类器官或单层细胞,是用于肠道模型的有力工具;
  • 从iPSC获得的肠细胞样细胞比Caco-2细胞(肠道模型的“金标准”)更像原代肠上皮细胞。
主编推荐语
mildbreeze
药物研发离不开细胞实验,比如用不同细胞系作为体外肠道模型来评估药物吸收和代谢。随着诱导多能干细胞(iPSC)的出现和分化培养条件的优化,用iPSC分化为肠上皮样细胞作为肠道模型,将在药物研发中发挥大作用。Trends in Molecular Medicine专门发表综述,值得专业人士关注。
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iPSC-Derived Enterocyte-like Cells for Drug Absorption and Metabolism Studies

用于药物吸收和代谢研究的iPSC衍生的肠细胞样细胞

10.1016/j.molmed.2018.06.001

2018-06-23, Review

Abstract & Authors:展开

Abstract:收起
Intestinal cell models have been widely studied and used to evaluate absorption and metabolism of drugs in the small intestine, constituting valuable tools as a first approach to evaluate the behavior of new drugs. However, such cell models might not be able to fully predict the absorption mechanisms and metabolic pathways of the tested compounds. In recent years, induced pluripotent stem cells (iPSCs) differentiated into enterocyte-like cells have been proposed as more biorelevant intestinal models. In this review, we describe mechanisms underlying the differentiation of iPSCs into enterocyte-like cells, appraise the usefulness of these cells in tridimensional intestinal models, and discuss their suitability to be used in the future for drug screening.

First Authors:
Maria Helena Macedo

Correspondence Authors:
Bruno Sarmento

All Authors:
Maria Helena Macedo,Francisca Araújo,Elena Martínez,Cristina Barrias,Bruno Sarmento

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