多组学分析结直肠癌特异性外泌体成分
创作:Lexi 审核:Lexi 2019年11月11日
  • 从结直肠癌(CRC)患者和健康对照(HC)血液中分离并鉴定了四种miRNA(mi-R19a、miR-21、miR-92a和miR-1246)阳性外泌体;
  • 氨酰tRNA生物合成途径在CRC中发生改变;
  • 脂肪酸代谢在CRC中大量增加,鞘脂类和甘油磷脂代谢在CRC中变化最显著;
  • 发生显著改变的蛋白有SYNE1、A2M、CACNA2D2、H2AFJ、胎儿类固醇结合蛋白、鞘磷脂合成酶2等;
  • 发生改变的联合通路有不饱和脂肪酸合成、苯丙氨酸-酪氨酸-色氨酸合成、氨酰tRNA合成途径等。
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Lexi
外泌体是自然分泌的由生化分子(包括RNA、代谢产物、脂质和蛋白质等)组成的纳米囊泡,可作为诊断工具和疾病特异性报告。组学技术近年来较为流行,并常用来发现并鉴定外泌体成分。最新发表在Cancer Letters的一项研究,利用组学技术建立了一个系统方法,可同时分析在结直肠癌中发生改变的代谢产物、脂类和蛋白质。该研究发现,与健康对照相比,在结直肠癌中,不饱和脂肪酸合成、苯丙氨酸-酪氨酸-色氨酸合成、氨酰tRNA合成途径发生显著改变。该研究有助于发现低丰度特征成分,并解释结直肠癌的分子基础。
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Cancer Letters [IF:6.508]

Untargeted Multi-Omic Analysis of Colorectal Cancer-Specific Exosomes Reveals Joint Pathways of Colorectal Cancer in both Clinical Samples and Cell Culture

结直肠癌特异性外泌体的非靶向多组学分析揭示了结直肠癌在临床样本和细胞培养中的联合通路

10.1016/j.canlet.2019.10.038

2019-10-25, Article

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Abstract Exosomes are naturally secreted nano-vesicles consisting of biochemical molecules including RNAs, metabolites, lipids, and proteins, that emerge as diagnostic tools and disease-specific reporters. Here we offer a systematic and integrative approach for the simultaneous analysis of altered molecules namely metabolites, lipids, and proteins. These components tend to augment the discovery of low abundance signature components, and assist in explanation of molecular basis of colorectal cancer (CRC). In order to investigate CRC-derived exosomes, we selected mi-R19a, miR-21, miR-92a, and miR-1246 positive exosomes for downstream experiments. The overall multi-omic changes were investigated comparatively in cell culture and serum samples. Following a systematic multi-omic study, 37 (cell culture) and 31 (serum) metabolites; 130 (cell culture) and 56 (serum) lipids; 9 (cell culture) and 13 (serum) proteins were seen to be differentially expressed (p<0.05), enabling discrimination between CRC and control. By using these enriched components, we demonstrated that the joint pathways mainly involving fatty acid and amino acid metabolism related pathways changed in CRC significantly. We conclude that this study increases our understanding of molecular basis of CRC, and provides potential exosomal biomarkers for the non-invasive detection, and discrimination of CRC.

First Authors:
Cemil Can Eylem

Correspondence Authors:
Burak Derkus

All Authors:
Cemil Can Eylem,Mehmet Yilmaz,Burak Derkus,Emirhan Nemutlu,Can Berk Camci,Erkan Yilmaz,Mehmet Akif Turkoglu,Bulent Aytac,Neslihan Ozyurt,Emel Emregul

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