吕农华等:肠道菌群失衡可加剧急性胰腺炎
  • 分析130例急性胰腺炎(AP)患者+35例健康人的粪便16S rRNA多样性,发现二者肠道菌群结构存在显著差异,且与全身炎症反应和肠屏障受损密切相关;
  • 肠道菌群组成随病情加重进一步改变,重症患者中Blautia等有益菌丰度明显少于轻症和中度重症患者;
  • 功能预测显示AP细菌侵袭上皮细胞通路活性升高,且与Escherichia-Shigella丰度呈正相关;
  • 与正常小鼠相比,假无菌小鼠和无菌小鼠诱导AP后胰腺损伤更轻,而移植正常小鼠粪便可加重胰腺病变。
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mildbreeze
来自南昌大学第一附属医院吕农华教授团队的研究,首先通过比较急性胰腺炎(AP)患者和正常人肠道菌群结构,发现AP早期存在肠道菌群失衡且与疾病严重程度有关;进一步利用动物模型证实肠道菌群失衡可能在AP发生发展中发挥重要作用,与之前一篇肠道菌群与胰腺疾病的综述(查看文章)提出的观点不谋而合,为未来调节肠道菌群治疗胰腺疾病提供新思路。
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Gut microbiota dysbiosis worsens the severity of acute pancreatitis in patients and mice

肠道菌群失调使患者和小鼠急性胰腺炎病情加重

10.1007/s00535-018-1529-0

2018-12-05, Article

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BACKGROUND: The gut is implicated in the pathogenesis of acute pancreatitis (AP) and the infectious complications of AP are commonly associated with enteric bacteria, yet whether gut microbiota dysbiosis participants in AP severity remains largely unknown.
METHODS: We collected clinical information and fecal samples from 165 adult participants, including 41 with mild AP (MAP), 59 with moderately severe AP (MSAP), 30 with severe AP (SAP) and 35 healthy controls (HC). The serum inflammatory cytokines and gut barrier indexes were detected. Male C57BL/6 mice with AP were established and injuries of pancreas were evaluated in antibiotic-treated mice, germ-free mice as well as those transplanted with fecal microbiota. The gut microbiota was analyzed by 16S rRNA gene sequencing.
RESULTS: The structure of gut microbiota was significantly different between AP and HC, and the disturbed microbiota was closely correlated with systematic inflammation and gut barrier dysfunction. Notably, the microbial composition changed further with the worsening of AP and the abundance of beneficial bacteria such as Blautia was decreased in SAP compared with MAP and MSAP. The increased capacity for the inferred pathway, bacterial invasion of epithelial cells in AP, highly correlated with the abundance of Escherichia-Shigella. Furthermore, the antibiotic-treated mice and germ-free mice exhibited alleviated pancreatic injury after AP induction and subsequent fecal microbiota transplantation in turn exacerbated the disease.
CONCLUSIONS: This study identifies the gut microbiota as an important mediator during AP and its dysbiosis is associated with AP severity, which suggests its role as potential therapeutic target.

First Authors:
Yin Zhu,Cong He,Xueyang Li

Correspondence Authors:
Nonghua Lu

All Authors:
Yin Zhu,Cong He,Xueyang Li,Yan Cai,Jinxiang Hu,Yuanhang Liao,Jianhua Zhao,Liang Xia,Wenhua He,Linmeng Liu,Chun Luo,Xu Shu,Qiang Cai,Youxiang Chen,Nonghua Lu

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