合生制剂改善食源性肥胖小鼠的代谢及菌群
创作:海盗船长 审核:szx 2020年02月21日
  • 高脂饮食小鼠分别用益生菌(动物双歧杆菌乳亚种DSM10140和副干酪乳杆菌干酪亚种DSM46331)、益生元(燕麦β-葡聚糖)或合生制剂(上述混合物)干预;
  • 合生制剂可显著减少体重增加,改善血糖、血脂等指标,逆转高脂饮食引发的肠道菌群变化,上述作用可能由特定分类群所介导;
  • 合生制剂可能通过影响碳水化合物、氨基酸和能量代谢重建肠道稳态;
  • 所有饮食干预均能恢复盲肠的乙酸、丙酸和丁酸水平,合生制剂可最有效地降低胆汁酸水平。
主编推荐语
周旸
Molecular Metabolism上发表的一项研究,使用益生菌、益生元及合生制剂干预高脂饮食喂养的小鼠,发现合生制剂可改善多项代谢指标,且其代谢改善作用与其对肠道菌群的调节相关。
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Synbiotic-driven improvement of metabolic disturbances is associated with changes in the gut microbiome in diet-induced obese mice

合生制剂对食源性肥胖小鼠代谢紊乱的改善作用与肠道菌群变化相关

10.1016/j.molmet.2019.01.012

2019-02-06, Article

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Objective: The gut microbiota is an important influencing factor of metabolic health. Although dietary interventions with probiotics, prebiotics, and synbiotics can be effective means to regulate obesity and associated comorbidities, the underlying shifts in gut microbial communities, especially at the functional level, have not been characterized in great details. In this study, we sought to investigate the effects of synbiotics on the regulation of gut microbiota and the alleviation of high-fat diet (HFD)-induced metabolic disorders in mice.
Methods: Specific pathogen-free (SPF) male C57BL/6J mice were fed diets with either 10% (normal diet, ND) or 60% (high-fat diet, HFD) of total calories from fat (lard). Dietary interventions in the HFD-fed mice included (i) probiotic (Bifidobacterium animalis subsp. lactis DSM 10140 and Lactobacillus paracasei subsp. paracasei DSM 46331), (ii) prebiotic (oat β-glucan), and (iii) synbiotic (a mixture of i and ii) treatments for 12 weeks. Besides detailed characterization of host metabolic parameters, a multi-omics approach was used to systematically profile the microbial signatures at both the phylogenetic and functional levels using 16S rRNA gene sequencing, metaproteomics and targeted metabolomics analysis.
Results: The synbiotic intervention significantly reduced body weight gain and alleviated features of metabolic complications. At the phylogenetic level, the synbiotic treatment significantly reversed HFD-induced changes in microbial populations, both in terms of richness and the relative abundance of specific taxa. Potentially important species such as Faecalibaculum rodentium and Alistipes putredinis that might mediate the beneficial effects of the synbiotic were identified. At the functional level, short-chain fatty acid and bile acid profiles revealed that all dietary interventions significantly restored cecal levels of acetate, propionate, and butyrate, while the synbiotic treatment reduced the bile acid pools most efficiently. Metaproteomics revealed that the effects of the synbiotic intervention might be mediated through metabolic pathways involved in carbohydrate, amino acid, and energy metabolisms.
Conclusions: Our results suggested that dietary intervention using the novel synbiotic can alleviate HFD-induced weight gain and restore gut microbial ecosystem homeostasis phylogenetically and functionally.

First Authors:
Xinxin Ke

Correspondence Authors:
Peter CK Cheung

All Authors:
Xinxin Ke,Alesia Walker,Sven-Bastiaan Haange,Ilias Lagkouvardos,Yuwen Liu,Philippe Schmitt-Kopplin,Martin von Bergen,Nico Jehmlich,Xin He,Thomas Clavel,Peter CK Cheung

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