FAF1 phosphorylation by AKT accumulates TGF-β type II receptor and drives breast cancer metastasis
Abstract & Authors:展开
TGF-β is pro-metastatic for the late-stage breast cancer cells. Despite recent progress, the regulation of TGF-β type II receptor remains uncertain. Here we report that FAF1 destabilizes TβRII on the cell surface by recruiting the VCP/E3 ligase complex, thereby limiting excessive TGF-β response. Importantly, activated AKT directly phosphorylates FAF1 at Ser 582, which disrupts the FAF1-VCP complex and reduces FAF1 at the plasma membrane. The latter results in an increase in TβRII at the cell surface that promotes both TGF-β-induced SMAD and non-SMAD signalling. We uncover a metastasis suppressing role for FAF1 through analyses of FAF1-knockout animals, various in vitro and in vivo models of epithelial-to-mesenchymal transition and metastasis, an MMTV-PyMT transgenic mouse model of mammary tumour progression and clinical breast cancer samples. These findings describe a previously uncharacterized mechanism by which TβRII is tightly controlled. Together, we reveal how SMAD and AKT pathways interact to confer pro-oncogenic responses to TGF-β.
Feng Xie,Ke Jin,Li Shao
Fangfang Zhou,Long Zhang
Feng Xie,Ke Jin,Li Shao,Yao Fan,Yifei Tu,Yihao Li,Bin Yang,Hans van Dam,Peter Ten Dijke,Honglei Weng,Steven Dooley,Shuai Wang,Junling Jia,Jin Jin,Fangfang Zhou,Long Zhang