四川师大:谁在为“一天一苹果,医生远离我”负责?
创作:兵兵 审核:兵兵 2020年12月06日
  • 研究利用根皮苷(PHZ)干预高脂膳食(HFD)喂养的C57BL/6J小鼠,时长12周;
  • PHZ可以有效降低小鼠体重增加,缓解胰岛素抗性,降低HFD引发的高水平血浆脂多糖(LPS);
  • 同时,PHZ缓解HFD诱发的肠道菌群紊乱,增加短链脂肪酸产生,抑制粪便LPS生成;
  • 将PHZ干预组小鼠粪便移植给HFD小鼠,小鼠的代谢紊乱状态得到改善,同时胰高血糖素样肽2(GLP-2)分泌增加,肠屏障完整性得以恢复;
  • PHZ通过肠道菌群-屏障轴干预HFD小鼠肥胖。
主编推荐语
兵兵
四川师范大学张晓喻团队研究成果。根皮苷是苹果中的一种二氢查耳酮类化合物。本研究利用根皮苷干预高脂膳食喂养的小鼠,探究其对于小鼠肥胖、胰岛素抗性、脂多糖等的影响,以及在此过程中,肠道菌群和肠道屏障的变化。干预结果显示,根皮苷可以缓解高脂膳食带来的肥胖、代谢紊乱,恢复菌群结构和肠屏障等。进一步采用粪菌移植实验发现,根皮苷带来的菌群改变移植到未干预小鼠体内同样具有上述功效。因此,本研究初步认为,根皮苷通过作用于肠道菌群-肠屏障轴,干预高脂膳食引发的小鼠肥胖。
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Gut Microbes [IF:7.74]

Phlorizin ameliorates obesity-associated endotoxemia and insulin resistance in high-fat diet-fed mice by targeting the gut microbiota and intestinal barrier integrity

根皮苷通过干预高脂膳食小鼠的肠道菌群和肠屏障完整性,缓解肥胖相关内毒素血症和胰岛素抗性

10.1080/19490976.2020.1842990

2020-11-23, Article

Abstract & Authors:展开

Abstract:收起
Phlorizin (PHZ) is one of phytonutrients in apples that contributes to the health-promoting effect implicated by the saying, ‘an apple a day keeps the doctor away’. PHZ was firstly identified as a competitive inhibitor of sodium-glucose co-transporters-2 (SGLT2); however, its low bioavailability makes it hard to fully explain its pharmacological mechanisms. This study aimed to investigate the ameliorating effect of PHZ on high-fat diet (HFD)-induced obesity via modulating the “gut microbiota-barrier axis”. Firstly, C57BL/6 J mice were fed a normal chow diet (NCD) or HFD coadministered with or without PHZ for 12 weeks. Our results showed that PHZ supplementation significantly reduced HFD-induced body weight gain (P < .001), alleviated metabolic disorders (MDs) like insulin resistance (P < .001) and elevation of serum lipopolysaccharides (LPS) (P < .001), attenuated HFD-induced gut microbiota alterations, enhanced short-chain fatty acids (SCFAs) production (P < .001), and inhibited fecal LPS production (P < .001). To investigate the role of the fecal microbiota in the observed beneficial effects, a fecal microbiota transplantation (FMT) experiment was performed by transplanting the feces of the four groups of mice (as donor mice) daily collected from the fourth week to a new batch of acclimatized HFD-fed mice. Our results confirmed that feeding the gut contents of the PHZ-modulated mice could attenuate HFD-induced MDs, accompanied by enhanced glucagon-like peptide 2 (GLP-2) secretion (P < .001) and restoration of HFD-induced damage in the gut epithelial barrier. This study has provided evidence that the “gut microbiota-barrier axis” was an alternative target for the anti-obesity effect of PHZ. This work has also provided an explanation for the high efficacy of PHZ despite the low bioavailability, and PHZ holds great potential to be developed as a functional food ingredient.

First Authors:
Xiaoyu Zhang,Jiang Chen

Correspondence Authors:
Xiaoyu Zhang

All Authors:
Xiaoyu Zhang,Jiang Chen,Kang Yi,Ling Peng,Jie Xie,Xun Gou,Tong Peng,Lin Tang

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