Nature Reviews:一文读懂肝脏内的细胞分区(综述)
创作:大力 审核:mildbreeze 2020年12月25日
  • 位于肝小叶中轴的肝细胞暴露于不同的微环境中,导致肝脏任务功能的空间分区;
  • 通过单细胞技术重构全局肝细胞转录组的区带模式,揭示了肝组织结构分区的原则,例如:向门脉周高氧区分配高能量要求的任务、对生物分子进行空间回收和“生产线布局”等;
  • 对肝细胞和相邻的非实质细胞进行测序,可以重建其他肝细胞类型的整体分区模式;
  • 肝脏区带可产生肝病理的分区模式;
  • 基因表达的区带性是肠、肾等代谢器官的一个显著特征。
主编推荐语
mildbreeze
肝小叶是肝脏的结构和功能单位,以中央静脉为中心,呈多面棱柱状。肝小叶中的细胞功能和基因表达并非均一性的,而是呈现多种区带化(zonation)特征。Nature Reviews Gastroenterology and Hepatology发表的这篇综述对此进行了详细介绍,值得专业人士参考。
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Spatial heterogeneity in the mammalian liver

哺乳动物肝脏的空间异质性

10.1038/s41575-019-0134-x

2019-04-01, Review

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Hepatocytes operate in highly structured repeating anatomical units termed liver lobules. Blood flow along the lobule radial axis creates gradients of oxygen, nutrients and hormones, which, together with morphogenetic fields, give rise to a highly variable microenvironment. In line with this spatial variability, key liver functions are expressed non-uniformly across the lobules, a phenomenon termed zonation. Technologies based on single-cell transcriptomics have constructed a global spatial map of hepatocyte gene expression in mice revealing that ~50% of hepatocyte genes are expressed in a zonated manner. This broad spatial heterogeneity suggests that hepatocytes in different lobule zones might have not only different gene expression profiles but also distinct epigenetic features, regenerative capacities, susceptibilities to damage and other functional aspects. Here, we present genomic approaches for studying liver zonation, describe the principles of liver zonation and discuss the intrinsic and extrinsic factors that dictate zonation patterns. We also explore the challenges and solutions for obtaining zonation maps of liver non-parenchymal cells. These approaches facilitate global characterization of liver function with high spatial resolution along physiological and pathological timescales.

First Authors:
Shani Ben-Moshe

Correspondence Authors:
Shalev Itzkovitz

All Authors:
Shani Ben-Moshe,Shalev Itzkovitz

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