国内团队:单细胞转录组分析揭示,消化系统是新冠病毒的潜在传播途径
创作:徐笑 审核:mildbreeze 04月10日
  • ACE2和TMPRSS2在细胞中的共表达,是新冠病毒进入细胞的关键;
  • 分析食管、胃粘膜、回肠、结肠上皮和肺的单细胞转录组数据,分别鉴定出14/10/10/10/25种细胞类型;
  • 肺泡2型细胞(AT2)是共表达ACE2和TMPRSS2的主要肺部细胞类型,或是病毒在肺部的主攻靶点;
  • ACE2和TMPRSS2也在食管上皮细胞和腺细胞以及回肠和结肠的吸收性肠细胞里共表达;
  • 这些共表达的细胞类型中,ACE2在回肠和结肠的表达相对较高;
  • 粪口传播是新冠病毒传播的潜在途径。
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mildbreeze
细胞受体ACE2和跨膜丝氨酸蛋白酶2(TMPRSS2),在新冠病毒(SARS-CoV-2)进入细胞的过程中有关键作用。Gut近期发表了第二军医大学、清华大学和同济大学医学院等多个团队的合作研究,通过分析ACE2和TMPRSS2的单细胞共表达模式,鉴定出新冠病毒在肺和消化系统中潜在靶细胞类型,表明除了肺部,消化系统也是病毒感染的潜在靶点。
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Gut [IF:19.819]

Digestive system is a potential route of COVID-19: an analysis of single-cell coexpression pattern of key proteins in viral entry process

消化系统是COVID-19的潜在传播途径:病毒进入过程关键蛋白的单细胞共表达模式分析

10.1136/gutjnl-2020-320953

04-02, Article

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Objective : Since December 2019, a newly identified coronavirus (severe acute respiratory syndrome coronavirus (SARS-CoV-2)) has caused outbreaks of pneumonia in Wuhan, China. SARS-CoV-2 enters host cells via cell receptor ACE II (ACE2) and the transmembrane serine protease 2 (TMPRSS2). In order to identify possible prime target cells of SARS-CoV-2 by comprehensive dissection of ACE2 and TMPRSS2 coexpression pattern in different cell types, five datasets with single-cell transcriptomes of lung, oesophagus, gastric mucosa, ileum and colon were analysed.
Design : Five datasets were searched, separately integrated and analysed. Violin plot was used to show the distribution of differentially expressed genes for different clusters. The ACE2-expressing and TMPRRSS2-expressing cells were highlighted and dissected to characterise the composition and proportion.
Results: Cell types in each dataset were identified by known markers. ACE2 and TMPRSS2 were not only coexpressed in lung AT2 cells and oesophageal upper epithelial and gland cells but also highly expressed in absorptive enterocytes from the ileum and colon. Additionally, among all the coexpressing cells in the normal digestive system and lung, the expression of ACE2 was relatively highly expressed in the ileum and colon.
Conclusion: This study provides the evidence of the potential route of SARS-CoV-2 in the digestive system along with the respiratory tract based on single-cell transcriptomic analysis. This finding may have a significant impact on health policy setting regarding the prevention of SARS-CoV-2 infection. Our study also demonstrates a novel method to identify the prime cell types of a virus by the coexpression pattern analysis of single-cell sequencing data.

First Authors:
Hao Zhang,Zijian Kang,Haiyi Gong,Da Xu

Correspondence Authors:
Jian Zhan,Tong Meng,Wang Zhou,Jianmin Liu,Huji Xu

All Authors:
Hao Zhang,Zijian Kang,Haiyi Gong,Da Xu,Jing Wang,Zhixiu Li,Zifu Li,Xinggang Cui,Jianru Xiao,Jian Zhan,Tong Meng,Wang Zhou,Jianmin Liu,Huji Xu

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