Cell子刊:共生细菌发酵产物改变肠细胞脂质代谢
  • 分别使用m-ICcl2肠细胞和鼠类模型证明共生菌种副干乳酪杆菌和大肠杆菌参与肠道脂质代谢调节;
  • 副干酪乳杆菌产生L-乳酸盐抑制肠上皮细胞分泌乳糜微粒,肠细胞吸收L-乳酸盐产生丙二酸单酰辅酶A,抑制脂质β氧化,促进脂质存储;
  • 大肠杆菌RB01产生乙酸盐抑制肠上皮细胞分泌乳糜微粒,乙酸盐被肠细胞吸收代谢为乙酰辅酶A和AMP,上调AMPK / PGC-1a / PPARa信号通路,促进脂质β氧化;
  • 本研究为开发特定益生菌及代谢物为治疗产品提供依据。
主编推荐语
潇洒小姐陈
已知肠道共生菌群参与调节肠道消化、吸收、储存和分泌膳食脂质,但特定共生菌种在其中所起的作用仍未知。Cell Host & Microbe的这篇文章证明了副干乳酪杆菌和大肠杆菌在肠道脂质代谢中的不同的调节机理,认为二者代谢产物L-乳酸盐和乙酸盐通过不同方式降低肠道细胞对脂质的分泌。虽然和之前无菌动物的结果不一致,但作者认为本实验选用的是较早定植肠道的两种菌种,体外体内实验结果一致,而且无菌动物本身并不一定适合复杂的菌群。也许未来使用或抑制L-乳酸盐、乙酸盐及其产生菌种,可作为治疗代谢疾病或营养不良症的治疗方案。
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Fermentation Products of Commensal Bacteria Alter Enterocyte Lipid Metabolism

共生细菌发酵产物改变肠细胞脂质代谢

10.1016/j.chom.2020.01.028

2020-02-25, Article

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Despite the recognized capacity of the gut microbiota to regulate intestinal lipid metabolism, the role of specific commensal species remains undefined. Here, we aimed to understand the bacterial effectors and molecular mechanisms by which Lactobacillus paracasei and Escherichia coli regulate lipid metabolism in enterocytes. We show that L-lactate produced by L. paracasei inhibits chylomicron secretion from enterocytes and promotes lipid storage by a mechanism involving L-lactate absorption by enterocytes, its conversion to malonyl-CoA, and the subsequent inhibition of lipid beta-oxidation. In contrast, acetate produced by E. coli also inhibits chylomicron secretion by enterocytes but promotes lipid oxidation by a mechanism involving acetate absorption by enterocytes, its metabolism to acetyl-CoA and AMP, and the subsequent upregulation of the AMPK/PGC-1α/PPARα pathway. Our study opens perspectives for developing specific bacteria- and metabolite-based therapeutic interventions against obesity, atherosclerosis, and malnutrition by targeting lipid metabolism in enterocytes.

First Authors:
João R Araújo

Correspondence Authors:
Philippe J Sansonetti

All Authors:
João R Araújo,Asmaa Tazi,Odile Burlen-Defranoux,Sophie Vichier-Guerre,Giulia Nigro,Hélène Licandro,Sylvie Demignot,Philippe J Sansonetti

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Science Signaling期刊

Microbial metabolites shape lipid metabolism

2020-04-14

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