Cell子刊:分析IgA-菌群互作,揭示独特的IBD相关细菌
创作:mildbreeze 审核:mildbreeze 01月08日
  • 用IgA-SEQ方法,在一个炎症性肠病(IBD)队列中分析肠道菌群的IgA包被模式,鉴定与疾病和治疗相关的细菌;
  • 相较于健康人,IBD患者中有43个细菌类群的IgA包被水平升高;
  • 其中包括8个相对丰度与健康人相似的类群,提示相较于分析细菌的相对丰度,IgA-SEQ能鉴定出独特的IBD相关细菌;
  • 接受抗TNFα治疗的患者中,菌群的IgA包被模式发生改变;
  • 颤螺菌属的IgA包被水平(而非相对丰度)可预测疾病进展,其包被水平升高与延迟实行切除术相关。
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mildbreeze
炎症性肠病(IBD)是宿主遗传和肠道菌群失调的共同作用结果。此前有研究报道,IBD患者中促炎细菌类群被分泌型免疫球蛋白IgA高度包被。Cell Host and Microbe发表的一项新研究在184名IBD患者和34名健康对照中,研究了菌群的IgA包被模式,揭示了IBD中存在的一类独特的IgA包被细菌类群,而这些细菌在相对丰度上与健康人无显著差异,并找出了可以预测疾病进展的潜在的菌群标志物。这些发现对于进一步揭示IBD中的免疫-菌群互作规律、寻找新的IBD预防和治疗靶点以及预测标志物,都有参考意义。
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Immunoglobulin A Targets a Unique Subset of the Microbiota in Inflammatory Bowel Disease

炎症性肠病中,免疫球蛋白A靶向菌群的一个独特亚群

10.1016/j.chom.2020.12.003

2020-12-31, Article

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Abstract:收起
The immunopathogenesis of inflammatory bowel disease (IBD) has been attributed to a combination of host genetics and intestinal dysbiosis. Previous work in a small cohort of IBD patients suggested that pro-inflammatory bacterial taxa are highly coated with secretory immunoglobulin IgA. Using bacterial fluorescence-activated cell sorting coupled with 16S rRNA gene sequencing (IgA-SEQ), we profiled IgA coating of intestinal microbiota in a large cohort of IBD patients and identified bacteria associated with disease and treatment. Forty-three bacterial taxa displayed significantly higher IgA coating in IBD compared with controls, including 8 taxa exhibiting differential IgA coating but similar relative abundance. Patients treated with anti-TNF-α therapies exhibited dramatically altered microbiota-specific IgA responses compared with controls. Furthermore, increased IgA coating of Oscillospira was associated with a delay in time to surgery. These results demonstrate that investigating IgA responses to microbiota can uncover potential disease-modifying taxa and reveal improved biomarkers of clinical course in IBD.

First Authors:
Jason M Shapiro,Marcel R de Zoete,Noah W Palm

Correspondence Authors:
Richard A Flavell,JC Clemente

All Authors:
Jason M Shapiro,Marcel R de Zoete,Noah W Palm,Yaro Laenen,Rene Bright,Meaghan Mallette,Kevin Bu,Agata A Bielecka,Fang Xu,Andres Hurtado-Lorenzo,Samir A Shah,Judy H Cho,Neal S LeLeiko,Bruce E Sands,Richard A Flavell,JC Clemente

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Cell Host and Microbe期刊

A Diamond in the Rough: IgA-Seq Signatures Stratify New Onset IBD

Svetlana Lima, Randy S. Longman,2021-01-13

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