张和平等:植物乳杆菌P-8在宿主体内的基因组变异
创作:szx 审核:szx 2018年05月15日
  • 研究益生菌植物乳杆菌P-8在健康人及大鼠体内的基因组变异情况;
  • 单剂量服用可使P-8在宿主肠道中存留4-5周,连续4周服用可使P-8存留最多17周;
  • 从粪便样本中分离出92株P-8衍生菌株,通过比较基因组分析检测到19个SNP,表现出核心基因组的中性进化特征;
  • 另外,在几乎一半的样本中,检测到1-3个质粒的丢失;
  • 并检测到609bp来自其它物种的23S rRNA同源片段。
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szx
来自内蒙古农业大学的张和平团队的最新研究,分析了益生菌菌株——植物乳杆菌P-8在宿主体内的基因组存留及基因组稳定性情况。在肠道环境下,植物乳杆菌P-8丢失了一些质粒,并可能获得了一些其它物种的遗传信息。
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Genomic variations in probiotic Lactobacillus plantarum P-8 in the human and rat gut

人体及大鼠肠道中益生菌植物乳杆菌P-8的基因组变异

10.3389/fmicb.2018.00893

2018-05-08, Article

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The effects of probiotics on host gastrointestinal health have become an area of particular interest in the field of probiotic research. However, the impact of the host intestinal environment on genomic changes in probiotic organisms remains largely unknown. To investigate, Lactobacillus plantarum P-8, a well-studied probiotic bacterium, was consumed by healthy human volunteers and rats. Then, the persistence and genomic stability of P-8 in the host gut were surveyed. qPCR results revealed that after the consumption of one dose, P-8 could be detected in the host gastrointestinal tract for 4–5 weeks. By contrast, after 4 successive weeks of consumption, P-8 could be detected for up to 17 weeks after consumption ceased. In total, 92 P-8 derived strains were isolated from fecal samples and their genomes were sequenced and analyzed. Comparative genomic analysis detected 19 SNPs, which showed the characteristics of neutral evolution in the core genome. In nearly half of samples (n = 39, 42%), the loss of one to three plasmids was observed. The frequent loss of plasmids indicated reductive evolution in the accessory genome under selection pressure within the gastrointestinal tract. We also observed a 609-bp 23S rRNA homologous fragment that may have been acquired from other species after intake. Our findings offer insight into the complex reactions of probiotics to the gut environment during survival in the host. The in vivo genomic dynamics of L. plantarum P-8 observed in this study will aid the commercial development of probiotics with more stable characteristics.

First Authors:
Yuqin Song,Qiuwen He

Correspondence Authors:
Yujun Cui,Heping Zhang

All Authors:
Yuqin Song,Qiuwen He,Jiachao Zhang,Jianmin Qiao,Haiyan Xu,Zhi Zhong,Wenyi Zhang,Zhihong Sun,Ruifu Yang,Yujun Cui,Heping Zhang

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