备孕期饮食,或可影响受孕情况
创作:Epi汪 审核:Epi汪 03月05日
  • 纳入10只雌性恒河猴,首先在低脂饮食喂养,而后进行高脂西式饮食(WSD)喂养,分别在2个时期中评估体外受精潜力;
  • 当饮食过度到WSD时期后,恒河猴体重和体脂增加,但是未观察到胰岛素水平的变化;
  • WSD时期卵泡液中IL-1拮抗蛋白、皮质醇/肾上腺皮质醇比值显著降低;
  • 摄入WSD尽管使分离成熟卵母细胞的可能性增加,但是观察到生成的胚泡数量减少44%;
  • WSD饮食与囊胚的基因失调、蛋白通道活性、线粒体功能等存在关联。
主编推荐语
Epi汪
女性的西式饮食,或称高脂饮食,与不良的妊娠结局有关,但这是源于饮食本身还是潜在的代谢功能障碍导致的尚不清楚。因此,本研究通过灵长类动物实验,发现短期高脂饮食摄入会产生卵泡内促炎微环境,这种微环境在没有大规模代谢改变的情况下,与着床前发育受损相关。
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Short-term Western-style diet negatively impacts reproductive outcomes in primates

短期西式饮食对灵长类动物的生殖结局产生负面影响

10.1172/jci.insight.138312

02-22, Article

Abstract & Authors:展开

Abstract:收起
A maternal Western-style diet (WSD) is associated with poor reproductive outcomes, but whether this is from the diet itself or underlying metabolic dysfunction is unknown. Here, we performed a longitudinal study using regularly cycling female rhesus macaques (n = 10) that underwent 2 consecutive in vitro fertilization (IVF) cycles, one while consuming a low-fat diet and another 6–8 months after consuming a high-fat WSD. Metabolic data were collected from the females prior to each IVF cycle. Follicular fluid (FF) and oocytes were assessed for cytokine/steroid levels and IVF potential, respectively. Although transition to a WSD led to weight gain and increased body fat, no difference in insulin levels was observed. A significant decrease in IL-1RA concentration and the ratio of cortisol/cortisone was detected in FF after WSD intake. Despite an increased probability of isolating mature oocytes, a 44% reduction in blastocyst number was observed with WSD consumption, and time-lapse imaging revealed delayed mitotic timing and multipolar divisions. RNA sequencing of blastocysts demonstrated dysregulation of genes involved in RNA binding, protein channel activity, mitochondrial function and pluripotency versus cell differentiation after WSD consumption. Thus, short-term WSD consumption promotes a proinflammatory intrafollicular microenvironment that is associated with impaired preimplantation development in the absence of large-scale metabolic changes.

First Authors:
Sweta Ravisankar

Correspondence Authors:
Jon D Hennebold

All Authors:
Sweta Ravisankar,Alison Y Ting,Melinda J Murphy,Nash Redmayne,Dorothy Wang,Carrie A McArthur,Diana L Takahashi,Paul Kievit,Shawn L Chavez,Jon D Hennebold

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