香港大学:当心新冠肺炎病毒的粪口传播
创作:orchid 审核:EADGBE 2020年06月02日
  • 全基因组序列分析显示,SARS-CoV-2与中华菊头蝠中鉴定出的SARS相关冠状病毒高度同源;
  • 蝙蝠肠道类器官对SARS-CoV-2完全易感,并能维持强大的病毒复制能力;
  • 随着时间的推移,在蝙蝠和人的小肠培养基中观察到病毒载量大大增加;
  • 部分COVID-19患者出现胃肠道症状,粪便标本中检测到病毒RNA,提示SARS-CoV-2除引起呼吸道感染,还可引起肠道感染;
  • SARS-CoV-2在人体肠道器官中复制旺盛,揭示人体肠道可能是SARS-CoV-2的传播途径之一。
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EADGBE
2019年12月新型冠状病毒(SARS-CoV-2)在中国武汉出现。截至2020年5月15日,2019年冠状病毒疾病确诊病例数(COVID-19)已超过440万(注:新冠病毒命名为SARS-CoV-2,新冠肺炎疾病命名为COVID-19)。SARS-CoV-2与2003年引起非典疫情的严重急性呼吸综合征冠状病毒(SARS-CoV)具有遗传相似性,两者属于同一个分支(clade)。中华菊头蝠(Rhinolophus sinicus)被认为是SARS-CoV的天然宿主。然而由于缺少可用于研究蝙蝠病毒的实验室模型,蝙蝠冠状病毒的研究受到了很大的局限。《Nature Medicine》上的一项研究发现,SARS-CoV-2可在蝙蝠和人肠道类器官中定植和复制,且在部分冠状病毒患者粪便中检测到病毒RNA,表明人体肠道可能是SARS-CoV-2的传播途径之一。但根据现有证据,粪口传播似乎并不是新冠肺炎传播的主要传播方式,其在新冠肺炎病毒传播中的地位和作用仍有待进一步研究。
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Nature Medicine [IF:36.13]

Infection of bat and human intestinal organoids by SARS-CoV-2

新冠病毒(SARS-CoV-2)对蝙蝠和人体肠道类器官的感染

10.1038/s41591-020-0912-6

2020-05-13, Other

Abstract & Authors:展开

Abstract:收起
A novel coronavirus—severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—emerged in humans in Wuhan, China, in December 2019 and has since disseminated globally1,2. As of April 16, 2020, the confirmed case count of coronavirus disease 2019 (COVID-19) had surpassed 2 million. Based on full-genome sequence analysis, SARS-CoV-2 shows high homology to SARS-related coronaviruses identified in horseshoe bats1,2. Here we show the establishment and characterization of expandable intestinal organoids derived from horseshoe bats of the Rhinolophus sinicus species that can recapitulate bat intestinal epithelium. These bat enteroids are fully susceptible to SARS-CoV-2 infection and sustain robust viral replication. Development of gastrointestinal symptoms in some patients with COVID-19 and detection of viral RNA in fecal specimens suggest that SARS-CoV-2 might cause enteric, in addition to respiratory, infection3,4. Here we demonstrate active replication of SARS-CoV-2 in human intestinal organoids and isolation of infectious virus from the stool specimen of a patient with diarrheal COVID-19. Collectively, we established the first expandable organoid culture system of bat intestinal epithelium and present evidence that SARS-CoV-2 can infect bat intestinal cells. The robust SARS-CoV-2 replication in human intestinal organoids suggests that the human intestinal tract might be a transmission route of SARS-CoV-2.

First Authors:
Jie Zhou,Cun Li,Xiaojuan Liu

Correspondence Authors:
Jie Zhou,Kwok Yung Yuen

All Authors:
Jie Zhou,Cun Li,Xiaojuan Liu,Man Chun Chiu,Xiaoyu Zhao,Dong Wang,Yuxuan Wei,Andrew Lee,Anna Jinxia Zhang,Hin Chu,Jian-Piao Cai,Cyril Chik-Yan Yip,Ivy Hau-Yee Chan,Kenneth Kak-Yuen Wong,Owen Tak-Yin Tsang,Kwok-Hung Chan,Jasper Fuk-Woo Chan,Kelvin Kai-Wang To,Honglin Chen,Kwok Yung Yuen

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