Science子刊:利用恒河猴模型开发弯曲杆菌疫苗
创作:aluba 审核:aluba 2020年07月03日
  • 野外饲养的恒河猴可自发弯曲杆菌相关腹泻(7年间的累积发病率可达16.2%),可作为弯曲杆菌疫苗研究的动物模型;
  • 基于缺失neuA/B/C基因及唾液酸转移酶基因的大肠弯曲杆菌,利用3%的过氧化氢及Cu2+开发灭活弯曲杆菌疫苗;
  • 弯曲杆菌疫苗可诱导恒河猴产生大量抗弯曲杆菌抗体,这些抗体可靶向包括鞭毛蛋白在内的多种弯曲杆菌蛋白;
  • 接种后随访1年,弯曲杆菌疫苗可使弯曲杆菌相关腹泻的发病率降低83%。
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aluba
大肠弯曲杆菌及空肠弯曲杆菌感染是全球范围内导致细菌性胃肠炎的最常见因素,但动物模型的缺乏导致弯曲杆菌的疫苗开发难以进行。《Science Advances》上发表的一项最新研究,利用野外饲养的恒河猴作为自发性弯曲杆菌相关腹泻模型,开发了一种新型弯曲杆菌疫苗,可诱导恒河猴体内产生大量抗弯曲杆菌抗体,并对弯曲杆菌相关腹泻提供多达83%的保护作用。
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Science Advances [IF:14.136]

Vaccine-mediated protection against Campylobacter-associated enteric disease

疫苗介导的对弯曲杆菌相关肠病的保护作用

10.1126/sciadv.aba4511

2020-06-24, Article

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Campylobacter coli and Campylobacter jejuni are responsible for 400 million to 500 million cases of enteric disease each year and represent the most common cause of bacterial gastroenteritis worldwide. Despite its global importance, Campylobacter vaccine development has been hampered by the lack of animal models that recapitulate human disease pathogenesis. Here, we describe a naturally occurring Campylobacter-associated diarrhea model in outdoor-housed rhesus macaques. Using this model, we developed novel next-generation H2O2-based Campylobacter vaccines that induced strong antibacterial antibodies to multiple Campylobacter proteins including flagellin and provided up to 83% protection against severe C. coli–associated diarrhea. Whole-genome sequencing of circulating Campylobacter strains revealed little to no homology within lipooligosaccharide or capsular polysaccharide loci with the Campylobacter vaccine strains used in these studies, indicating that vaccine-mediated immunity was not restricted to a single homologous serotype. Together, these results demonstrate an important advance in vaccine development and a new approach to reducing Campylobacter-associated enteric disease.

First Authors:
Benjamin K Quintel

Correspondence Authors:
Mark K Slifka,Ian J Amanna

All Authors:
Benjamin K Quintel,Kamm Prongay,Anne D Lewis,Hans-Peter Raué,Sara Hendrickson,Nicholas S Rhoades,Ilhem Messaoudi,Lina Gao,Mark K Slifka,Ian J Amanna

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Medical Xpress新闻网站

New vaccine holds promise in fighting diarrheal disease

2020-06-24

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