牛瘤胃菌群组成与维生素B12浓度相关
创作:this little piggy 审核:nana 03月18日
  • 检测50只不同泌乳期奶牛瘤胃、粪便、血液和牛奶的维生B12(VB12)浓度及瘤胃、粪便和牛奶的菌群;
  • 血液VB12浓度可预测牛奶中VB12浓度;
  • 瘤胃、粪便和牛奶的菌群结构不同,饲料组成和泌乳状态对瘤胃和粪便菌群组成影响显著;
  • 仅发现瘤胃的VB12浓度与其菌群相关,与普雷沃氏菌属丰度正相关,与拟杆菌、瘤胃梭菌属、丁酸弧菌属和琥珀酸弧菌科丰度负相关;
  • 瘤胃菌群对VB12的消耗或比合成更重要,瘤胃菌群或是提高VB12产量的新靶点。
主编推荐语
nana
维生素B12在奶牛的瘤胃中由微生物合成,人类通过食用乳制品来获取维生素B12。然而,牛奶中维生素B12的浓度变化很大,研究者对其中的原因尚不清楚。mSystems最近的研究,检测了荷斯坦奶牛瘤胃、粪便和牛奶的微生物组成,并试图了解每个部位的微生物群落是如何影响维生素B12在奶牛体内的产生和运输的。结果表明,牛瘤胃微生物组的组成与维生素B12的浓度有很强的相关性,说明瘤胃微生物群可能是提高维生素B12产量的潜在调控靶点。
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mSystems [IF:6.633]

Correlations between the Composition of the Bovine Microbiota and Vitamin B12 Abundance

牛菌群组成与维生素B12浓度的关系

10.1128/mSystems.00107-20

03-03, Article

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Vitamin B12 is synthesized by prokaryotes in the rumens of dairy cows—and this has implications in human nutrition since humans rely on consumption of dairy products for vitamin B12 acquisition. However, the concentration of vitamin B12 in milk is highly variable, and there is interest in determining what causes vitamin B12 variability. We collected 92 temporally linked rumen, fecal, blood, and milk sample sets from Holstein cows at various stages of lactation fitted with rumen cannula and attempted to define which bacterial genera correlated well with vitamin B12 abundance. The level of vitamin B12 present in each sample was measured, and the bacterial population of each rumen, fecal, and milk sample (n = 263) was analyzed by 16S rRNA-targeted amplicon sequencing of the V4 region. The bacterial populations present in the rumen, small intestine, and milk were highly dissimilar. Combined diet and lactation status had significant effects on the composition of the microbiota in the rumen and in the feces. A high ruminal concentration of vitamin B12 was correlated with the increased abundance of Prevotella, while a low ruminal concentration of vitamin B12 was correlated with increased abundance of Bacteroidetes, Ruminiclostridium, and Butyrivibrio. The ultimate concentration of vitamin B12 is controlled by the complex interaction of several factors, including the composition of the microbiota. Bacterial consumption of vitamin B12 in the rumen may be more important in determining overall levels than bacterial production. IMPORTANCE In this paper, we examined the microbiome of the bovine rumen, feces, and milk and attempted to understand how the bacterial communities at each site affected the production and movement of vitamin B12 around the animal’s body. It was determined that the composition of the bovine rumen microbiome correlates well with vitamin B12 concentration, indicating that the rumen microbiota may be a good target for manipulation to improve production of this important vitamin.

First Authors:
Julian Franco-Lopez

Correspondence Authors:
Jennifer Ronholm

All Authors:
Julian Franco-Lopez,Melissa Duplessis,An Bui,Coralie Reymond,William Poisson,Lya Blais,Jasmine Chong,Rachel Gervais,Daniel E Rico,Roger I Cue,Christiane L Girard,Jennifer Ronholm

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